Abstract
Increased interleukin (IL)-8 plays an important role not only in activation and recruitment of neutrophils but also in inducing exaggerated angiogenesis at the inflamed site. In the present study, we investigated the fact that clotrimazole (CLT) inhibits intestinal inflammation, and the inhibitory action is mediated through suppression of IL-8 expression. In the trinitrobenzene sulfonic acid (TNBS)-induced rat colitis model, CLT dose-dependently protected from the TNBS-induced weight loss, colon ulceration, and myeloperoxidase activity increase. In the lesion site, CLT also suppressed the TNBS-induced angiogenesis, IL-8 expression, and nuclear factor (NF)-kappaB activation. In a cellular model of colitis using tumor necrosis factor (TNF)-alpha-stimulated HT29 colon epithelial cells, treatment with CLT significantly suppressed TNF-alpha-mediated IL-8 induction and NF-kappaB transcriptional activity revealed by a luciferase reporter gene assay. Furthermore, cotreatment with CLT and pyrrolidine dithiocarbamate, a NF-kappaB inhibitor, synergistically reduced the NF-kappaB transcriptional activity as well as IL-8 expression. In an in vitro angiogenesis assay, CLT suppressed IL-8-induced proliferation, tube formation, and invasion of...Continue Reading
References
Dec 11, 1992·Science·A E KochR M Strieter
Mar 1, 1982·The Journal of Investigative Dermatology·P P BradleyG Rothstein
Oct 13, 1995·Science·R I ScheinmanA S Baldwin
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Sep 1, 1996·Nature Medicine·M F NeurathW Strober
Feb 1, 1996·Gut·R DaigV Gross
Jul 1, 1997·Journal of Virology·T MurayamaK Matsushima
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·T S Blackwell, J W Christman
Mar 7, 1998·The Journal of Biological Chemistry·A R BrasierR P Garofalo
Apr 16, 1998·The Journal of Clinical Investigation·C WahlR M Schmid
Jun 9, 1998·Japanese Journal of Cancer Research : Gann·H TakahashiY Sato
Jul 3, 1998·Gastroenterology·C Fiocchi
Jul 25, 1998·Gastroenterology·G RoglerV Gross
Dec 13, 2000·The Journal of Pathology·E J WilliamsN Sheron
Feb 14, 2002·Trends in Molecular Medicine·Jonathan M Rhodes, Barry J Campbell
Dec 11, 2002·The Journal of Pathology·Charmian BanksNick Sheron
Dec 24, 2002·The Journal of Biological Chemistry·Jan HeidemannDavid G Binion
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aihua LiRakesh K Singh
Apr 4, 2003·Journal of Gastroenterology·Shinsuke SaitoHirokazu Nagawa
Apr 6, 2004·Developmental and Comparative Immunology·Kerry J Laing, Christopher J Secombes
May 15, 2004·The Journal of Biological Chemistry·Michael YehJudith A Berliner
Dec 8, 2004·Cancer Investigation·Andrezza V BeloSilvia P Andrade
Dec 14, 2004·The Journal of Biological Chemistry·Sunil K Manna, Govindarajan T Ramesh
Jun 16, 2005·Journal of Gastroenterology and Hepatology·Hua-Tian GanQin Ouyang
Aug 27, 2005·Journal of Neurosurgery·M Humayun KhalidEric Walters
Sep 1, 2005·Angiogenesis·Aihua LiRakesh K Singh
Dec 24, 2005·The Journal of Pharmacology and Experimental Therapeutics·Naoki KobayashiTeruna J Siahaan
Dec 16, 2006·Gut·Silvio DaneseClaudio Fiocchi
Mar 9, 2007·European Journal of Pharmacology·Marina Sánchez-HidalgoCatalina Alarcón de la Lastra
Apr 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·John H ChidlowChristopher G Kevil
May 10, 2007·The Journal of Pharmacology and Experimental Therapeutics·Luca AntonioliMario Del Taca
Oct 18, 2007·Inflammatory Bowel Diseases·Sreedhar SubramanianBarry J Campbell
Jan 30, 2008·European Journal of Clinical Investigation·I D PousaJ P Gisbert
Citations
Oct 12, 2012·Investigational New Drugs·Christian FreiseRajan Somasundaram
Jul 31, 2013·Inflammation·Arif Burak CekicCengiz Erçin
May 2, 2009·Archives of Pharmacal Research·Dinesh ThapaJung-Ae Kim
Aug 7, 2012·Archives of Pharmacal Research·Prabagar BalakrishnanHan-Gon Choi
Sep 21, 2011·Infection and Immunity·Jin-Hyung LeeJintae Lee
Nov 21, 2012·Journal of Ethnopharmacology·Wenna ZhaoWenji Sun
Mar 8, 2010·Pharmaceuticals·Domenico Ribatti
Sep 17, 2009·The American Journal of Gastroenterology·Udayakumar Navaneethan, Bo Shen
Jul 31, 2013·Antimicrobial Agents and Chemotherapy·Duncan WilsonBernhard Hube
Jan 30, 2016·PloS One·Suhrid BanskotaJung-Ae Kim
Nov 3, 2010·Alimentary Pharmacology & Therapeutics·S SamuelW J Sandborn
Aug 16, 2012·British Journal of Pharmacology·D StrøbækP Christophersen
Oct 18, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Chhabi Lal ChaudharyByeong-Seon Jeong
May 6, 2020·International Journal of Molecular Sciences·Pallavi GurungByeong-Seon Jeong
Apr 16, 2010·Biology of Reproduction·Nicoletta Di SimoneRiccardo Marana
Aug 29, 2018·MedChemComm·Sang Won ParkTae-Gyu Nam
Aug 4, 2020·Bioorganic Chemistry·Chhabi Lal ChaudharyByeong-Seon Jeong
Sep 22, 2009·European Journal of Pharmacology·Kyoung-Jin KimYoung-Joon Park
May 12, 2009·Developmental and Comparative Immunology·Xiuxia ZhouHeping Dai
May 3, 2011·Developmental and Comparative Immunology·Xianwei CuiShuangquan Zhang
Mar 14, 2012·Chemico-biological Interactions·Su-Young ParkJung-Ae Kim