Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration

Cytotherapy
Mizuho KittakaHidemi Kurihara

Abstract

The transplantation of mesenchymal stromal cells (MSCs) to damaged tissue has attracted attention in scientific and medical fields as an effective regenerative therapy. Nevertheless, additional studies are required to develop an MSC transplant method for bone regeneration because the use of an artificial scaffold restricts the number of transplanted cells and their function. Furthermore, regulating the degree of cell differentiation in vitro is desirable for a more effective regenerative therapy. To address these unresolved issues, with the use of a self-produced extracellular matrix (ECM), we developed clumps of an MSC/ECM complex (C-MSCs). MSCs isolated from rat femur were cultured in growth medium supplemented with 50 μg/mL of ascorbic acid for 7 days. To obtain C-MSCs, confluent cells were scratched with the use of a micropipette tip to roll up the cellular sheet, which consisted of ECM produced by the MSCs. The biological properties of C-MSCs were assessed in vitro and their bone regenerative activity was tested by use of a rat calvarial defect model. Immunofluorescent confocal microscopic analysis revealed that type I collagen formed C-MSCs. Osteopontin messenger RNA expression and amount of calcium content were higher in...Continue Reading

References

Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S P BruderS Kadiyala
Apr 2, 1999·Science·M F PittengerD R Marshak
Mar 14, 2002·Biochemical and Biophysical Research Communications·Felicity R A J Rose, Richard O C Oreffo
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ranieri CanceddaRodolfo Quarto
Nov 19, 2003·Advanced Drug Delivery Reviews·Judith E O'Grady, Diana M Bordon
Nov 2, 2004·Journal of Periodontology·Hiroyuki KawaguchiHidemi Kurihara
Sep 1, 2007·Biomaterials·Joseph YangTeruo Okano
Nov 13, 2007·Advanced Drug Delivery Reviews·Eileen DawsonKrishnendu Roy
Jul 22, 2008·Journal of Bioscience and Bioengineering·Kyung-Min ChoiJung-Keug Park
Oct 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Céline Colnot
Mar 31, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·D J LeemingM A Karsdal
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew D TreiserPrabhas V Moghe
Mar 24, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·I V YannasP T C So
Nov 9, 2010·Cell Transplantation·Ru-Huei FuShinn-Zong Lin
Mar 19, 2011·Journal of Cellular Biochemistry·Paolo Bianco
Sep 10, 2011·Tissue Engineering. Part B, Reviews·Céline Colnot
Mar 29, 2012·Journal of Cellular and Molecular Medicine·Patcharakamon NooeaidAldo R Boccaccini
Jul 11, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Céline ColnotMelissa L Knothe Tate
Sep 4, 2012·Trends in Biotechnology·Susmita BoseAmit Bandyopadhyay
Jan 1, 2014·Tissue Engineering. Part B, Reviews·Ryosuke KurodaMasahiro Kurosaka
Jan 21, 2014·Biomaterials·AhRan KangSang-Hoon Lee

❮ Previous
Next ❯

Citations

Jan 23, 2016·Cytotherapy·Ramkumar Tiruvannamalai AnnamalaiJan P Stegemann
Nov 2, 2016·Connective Tissue Research·Puay Yong NeoJames Cho-Hong Goh
May 19, 2017·Journal of Dental Research·M TakewakiH Kurihara
Jan 24, 2020·Journal of Bone and Mineral Metabolism·Eri Kaneda-IkedaHidemi Kurihara
May 23, 2020·Plastic and Reconstructive Surgery. Global Open·Marie NaudotSophie Le Ricousse
Jun 3, 2021·International Journal of Molecular Sciences·Raphael Gorodetsky, Wilhelm K Aicher
Aug 8, 2021·Cells·Filip Simunovic, Günter Finkenzeller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.