Clustered intergenic region sequences as predictors of factor H Binding Protein expression patterns and for assessing Neisseria meningitidis strain coverage by meningococcal vaccines.

PloS One
Caroline CayrouChristopher D Bayliss

Abstract

Factor H binding protein (fHbp) is a major protective antigen in 4C-MenB (Bexsero®) and Trumenba®, two serogroup B meningococcal vaccines, wherein expression level is a determinant of protection. Examination of promoter-containing intergenic region (IGR) sequences indicated that nine fHbp IGR alleles covered 92% of 1,032 invasive meningococcal strains with variant 1 fHbp alleles. Relative expression values for fHbp were determined for 79 meningococcal isolates covering ten IGR alleles by quantitative reverse transcriptase polymerase chain reaction (qRT PCR). Derivation of expression clusters of IGR sequences by linear regression identified five expression clusters with five nucleotides and one insertion showing statistically associations with differences in expression level. Sequence analysis of 273 isolates examined by the Meningococcal Antigen Typing Scheme, a sandwich ELISA, found that coverage depended on the IGR expression cluster and vaccine peptide homology combination. Specific fHbp peptide-IGR expression cluster combinations were designated as 'at risk' for coverage by 4C-MenB and were detected in multiple invasive meningococcal disease cases confirmed by PCR alone and occurring in partially-vaccinated infants. We conc...Continue Reading

References

Mar 19, 2003·The Journal of Experimental Medicine·Vega MasignaniMariagrazia Pizza
Mar 21, 2006·Journal of Bacteriology·Isabel DelanyVincenzo Scarlato
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guillermo MadicoSanjay Ram
Jul 4, 2009·Microbiology·Peter T Beernink, Dan M Granoff
Oct 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·John DonnellyMarzia Monica Giuliani
Dec 15, 2010·BMC Bioinformatics·Keith A Jolley, Martin C J Maiden
Aug 14, 2012·Journal of Bacteriology·Chunxiao Yu, Caroline Attardo Genco
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jun 26, 2013·The Journal of Infection·Ellen HeinsbroekMary Ramsay
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 25, 2014·Pathogens and Global Health·George Vernikos, Duccio Medini
Oct 31, 2015·The Lancet Infectious Diseases·Dorothea M C HillMartin C J Maiden
Feb 13, 2016·Lancet·Christopher D BaylissRay Borrow
Feb 19, 2016·Proceedings of the National Academy of Sciences of the United States of America·Massimiliano BiaginiVega Masignani

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Methods Mentioned

BETA
Typing
PCR
electrophoresis
X-ray

Software Mentioned

ARNold
Fuzznuc (
MUSCLE
EMBOSS package
ImageJ
BioEdit
PubMLST

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