Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material

Investigative Ophthalmology & Visual Science
Matthias ZenkelUrsula Schlötzer-Schrehardt

Abstract

To determine the role of the extracellular chaperone clusterin in the pathophysiology of pseudoexfoliation (PEX) syndrome/glaucoma, which is characterized by the stable deposition of abnormal extracellular fibrillar material in anterior segment tissues. Real-time PCR, in situ hybridization, and immunohistochemistry were applied to analyze the mRNA and protein expression of clusterin in PEX eyes of patients without and with glaucoma and to compare them with eyes of patients with primary open-angle glaucoma and angle-closure glaucoma and with normal control eyes. Aqueous levels of clusterin were determined by Western blot analysis. Real-time PCR and Western blot analysis were used to study the effect of TGF-beta1, which is significantly increased in the aqueous humor of PEX eyes, on clusterin expression by nonpigmented ciliary epithelial cells in vitro. Clusterin mRNA was ubiquitously expressed in most ocular cells and tissues, particularly in the epithelium of ciliary processes, whereas the protein was mostly located to extracellular structures, such as ocular basement membranes and stromal fibers. Real-time PCR and in situ hybridization displayed significant downregulation of clusterin mRNA in all anterior segment tissues of PE...Continue Reading

Citations

Oct 12, 2012·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·U Schlötzer-Schrehardt
Sep 3, 2010·European Journal of Human Genetics : EJHG·Mandy KrumbiegelAndré Reis
Feb 28, 2008·Current Opinion in Ophthalmology·Richard K Lee
Jan 10, 2013·Current Opinion in Ophthalmology·Julia SeinCarol L Karp
Jun 15, 2010·Journal of Glaucoma·Mandy KrumbiegelAndré Reis
Aug 5, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Gordon W LaurieJason J Nichols
Nov 20, 2012·Human Genomics·Eman ElhawyJohn Danias
May 12, 2009·BMC Medical Genomics·Tatiana NikolskayaValery I Shestopalov
Oct 3, 2012·PloS One·Sarah F JanssenArthur A B Bergen
Apr 7, 2011·Clinical Ophthalmology·Oscar BuchacraOana Stirbu
May 17, 2011·Middle East African Journal of Ophthalmology·Ursula Schlötzer-Schrehardt
May 27, 2014·Japanese Journal of Ophthalmology·Emrullah BeyazyıldızFaruk Oztürk
Aug 5, 2009·Brain Research Reviews·Tapio NuutinenAntero Salminen
Oct 12, 2007·Experimental Eye Research·U Schlötzer-SchrehardtF E Kruse
Apr 11, 2007·Biochemical and Biophysical Research Communications·Tiina SuuronenAntero Salminen
Jul 12, 2013·European Journal of Oral Sciences·Qalb-E-Saleem KhanHarald Osmundsen
May 2, 2016·Experimental Eye Research·M Elizabeth FiniMark R Wilson
Aug 15, 2014·PloS One·Humaira AyubAnneke I den Hollander
Sep 24, 2008·Experimental Eye Research·Ursula Schlötzer-Schrehardt
Sep 10, 2014·Journal of Glaucoma·Sibel KocabeyogluJale Karakaya
Jan 1, 2012·European Journal of Mass Spectrometry·Amelie Botling TaubeJonas Bergquist
Feb 9, 2018·Journal of Glaucoma·Ursula Schlötzer-Schrehardt
Oct 4, 2018·European Journal of Ophthalmology·Luca TerraccianoRita Mencucci
Feb 16, 2019·Omics : a Journal of Integrative Biology·Varshasnata MohantyKrishna R Murthy
Apr 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Justin J YerburyMark R Wilson
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Mahdi GhaniEkaterina Rogaeva
Aug 30, 2014·International Ophthalmology Clinics·Luis E Vazquez, Richard K Lee
Jan 17, 2015·Current Opinion in Ophthalmology·Eleftherios AnastasopoulosFotis Topouzis
Sep 1, 2015·International Ophthalmology Clinics·Aliya Z Jiwani, Louis R Pasquale
Oct 3, 2014·Journal of Glaucoma·Matthias Zenkel, Ursula Schlötzer-Schrehardt
Jul 1, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Justus G GarwegIsabel B Pfister
Jul 26, 2019·Journal of Ophthalmology·Hanna LesiewskaGrażyna Malukiewicz
Nov 19, 2019·Acta Ophthalmologica·Mehdi Ghaffari SharafLarry D Unsworth
Apr 9, 2013·European Journal of Ophthalmology·Sibel KocabeyogluMehmet Orhan

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