Clusterin exerts a cytoprotective and antioxidant effect in human osteoarthritic cartilage.

Aging
Chiara TarquiniA Orlandi

Abstract

Osteoarthritis (OA) is the most common joint disease characterized by destruction of articular cartilage. OA-induced cartilage degeneration causes inflammation, oxidative stress and the hypertrophic shift of quiescent chondrocytes. Clusterin (CLU) is a ubiquitous glycoprotein implicated in many cellular processes and its upregulation has been recently reported in OA cartilage. However, the specific role of CLU in OA cartilage injury has not been investigated yet. We analyzed CLU expression in human articular cartilage in vivo and in cartilage-derived chondrocytes in vitro. CLU knockdown in OA chondrocytes was also performed and its effect on proliferation, hypertrophic phenotype, apoptosis, inflammation and oxidative stress was investigated. CLU expression was upregulated in human OA cartilage and in cultured OA cartilage-derived chondrocytes compared with control group. CLU knockdown reduced cell proliferation and increased hypertrophic phenotype as well as apoptotic death. CLU-silenced OA chondrocytes showed higher MMP13 and COL10A1 as well as greater TNF-α, Nox4 and ROS levels. Our results indicate a possible cytoprotective role of CLU in OA chondrocytes promoting cell survival by its anti-apoptotic, anti-inflammatory and an...Continue Reading

References

Mar 6, 1999·The Journal of Biological Chemistry·D T HumphreysM R Wilson
Apr 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·A OrlandiL G Spagnoli
Oct 3, 2000·Arthritis and Rheumatism·M B Goldring
Feb 14, 2002·Osteoarthritis and Cartilage·J R ConnorM W Lark
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Aug 30, 2002·The International Journal of Biochemistry & Cell Biology·Ioannis P Trougakos, Efstathios S Gonos
Jan 29, 2003·The Journal of Biological Chemistry·Konstantin S LeskovDavid A Boothman
Dec 1, 1957·Annals of the Rheumatic Diseases·J H KELLGREN, J S LAWRENCE
Dec 14, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Augusto OrlandiLuigi Giusto Spagnoli
Mar 16, 2005·Lancet·Paul A Dieppe, L Stefan Lohmander
Sep 16, 2005·Expert Review of Neurotherapeutics·Abhilash K Desai, George T Grossberg
Apr 20, 2006·The Journal of Pathology·D VarogaT Pufe
Nov 3, 2009·Advances in Cancer Research·Sabina Pucci, Saverio Bettuzzi
Nov 3, 2009·Advances in Cancer Research·Géraldine Falgarone, Gilles Chiocchia
Dec 17, 2009·American Journal of Physiology. Renal Physiology·Wenjun ZhouCaigan Du
Dec 24, 2009·The Journal of Biological Chemistry·Abdellatif EssabbaniGilles Chiocchia
Apr 16, 2010·Annals of the New York Academy of Sciences·Mary B Goldring, Steven R Goldring
Aug 12, 2010·Clinics in Geriatric Medicine·Yuqing Zhang, Joanne M Jordan
Jan 19, 2011·The Journal of Biological Chemistry·Stephanie MateriaSharon La Fontaine
Dec 2, 2011·The Journal of Biological Chemistry·Stephanie MateriaSharon La Fontaine
Dec 6, 2011·Amino Acids·Umberto TarantinoAugusto Orlandi
Dec 20, 2011·Osteoarthritis and Cartilage·P M van der Kraan, W B van den Berg
Jan 31, 2014·American Journal of Physiology. Renal Physiology·Christopher Y C NguanCaigan Du
Mar 29, 2014·OncoTargets and Therapy·Tomas Koltai
May 6, 2014·Best Practice & Research. Clinical Rheumatology·Victoria L Johnson, David J Hunter
May 31, 2014·Mediators of Inflammation·Piotr WojdasiewiczDariusz Szukiewicz
Oct 8, 2014·Arthritis Research & Therapy·Susan Y RitterAntonios O Aliprantis
Sep 10, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Libin XuDongmei Zhang
Jan 16, 2016·Biochimica Et Biophysica Acta·Panagiotis Lepetsos, Athanasios G Papavassiliou

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
biosensor
X-ray
light microscopy

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Valérie DevauchelleGilles Chiocchia
Biomolecular Concepts
Philipp RohneClaudia Koch-Brandt
Molecular Neurodegeneration
Aleksandra M WojtasJohn D Fryer
Expert Opinion on Therapeutic Targets
Mark R Wilson, Amina Zoubeidi
© 2022 Meta ULC. All rights reserved