Clusterin is an extracellular chaperone that specifically interacts with slowly aggregating proteins on their off-folding pathway

FEBS Letters
Stephen PoonJ A Carver

Abstract

Clusterin is an extracellular mammalian chaperone protein which inhibits stress-induced precipitation of many different proteins. The conformational state(s) of proteins that interact with clusterin and the stage(s) along the folding and off-folding (precipitation-bound) pathways where this interaction occurs were previously unknown. We investigated this by examining the interactions of clusterin with different structural forms of alpha-lactalbumin, gamma-crystallin and lysozyme. When assessed by ELISA and native gel electrophoresis, clusterin did not bind to various stable, intermediately folded states of alpha-lactalbumin nor to the native form of this protein, but did bind to and inhibit the slow precipitation of reduced alpha-lactalbumin. Reduction-induced changes in the conformation of alpha-lactalbumin, in the absence and presence of clusterin, were monitored by real-time (1)H NMR spectroscopy. In the absence of clusterin, an intermediately folded form of alpha-lactalbumin, with some secondary structure but lacking tertiary structure, aggregated and precipitated. In the presence of clusterin, this form of alpha-lactalbumin was stabilised in a non-aggregated state, possibly via transient interactions with clusterin prior t...Continue Reading

References

Oct 20, 1992·Biochimica Et Biophysica Acta·M R Wilson, S B Easterbrook-Smith
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R RudolphR Jaenicke
Oct 15, 1995·The Biochemical Journal·K P Das, W K Surewicz
Oct 25, 1995·Biochimica Et Biophysica Acta·J A CarverR J Truscott
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Kuwajima
Oct 23, 1997·Biochemical and Biophysical Research Communications·R J Ellis
Nov 22, 1997·FEBS Letters·Y C KudvaN L Eberhardt
Apr 16, 1998·Trends in Biochemical Sciences·W J Netzer, F U Hartl
Dec 16, 1998·European Journal of Biochemistry·R A LindnerJ A Carver
Mar 6, 1999·The Journal of Biological Chemistry·D T HumphreysM R Wilson
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P T Lansbury
Jul 30, 1999·Biochemical and Biophysical Research Communications·F A BettelheimJ S Zigler
Aug 17, 1999·Biochemical and Biophysical Research Communications·G J StegeW W de Jong
Mar 1, 2000·Trends in Biochemical Sciences·M R Wilson, S B Easterbrook-Smith
Mar 29, 2000·Seminars in Cell & Developmental Biology·R J Ellis
Aug 11, 2000·Microscopy Research and Technique·M CaleroJ Ghiso

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Citations

Jun 23, 2011·Age·Liliana MatosHenrique Almeida
Nov 15, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Karlheinz MannMaxwell T Hincke
Dec 6, 2011·Journal of Agricultural and Food Chemistry·Jiapei WangJianping Wu
Jun 19, 2009·The Journal of Biological Chemistry·Amy R WyattMark R Wilson
Dec 10, 2009·The Journal of Biological Chemistry·Amy R Wyatt, Mark R Wilson
Jan 28, 2014·PloS One·Rebecca A Dabbs, Mark R Wilson
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ruben VidalBernardino Ghetti
Oct 8, 2011·The Protein Journal·S Shirin ShahangianBijan Ranjbar
Jan 13, 2012·Cancer Investigation·A M Rodríguez-PiñeiroV S Martínez-Zorzano
Dec 18, 2015·Biomolecular Concepts·Philipp RohneClaudia Koch-Brandt
Dec 6, 2014·Biochemistry·Michael J GreeneLawreen H Connors
May 5, 2005·The European Journal of Neuroscience·Grzegorz K Wicher, Håkan Aldskogius
May 6, 2009·Histopathology·Ariane AigelsreiterHelmut Denk
Jun 8, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yong ZhuGuixing Qiu
Dec 25, 2010·Proteomics·Dileep A OmanaJianping Wu
Jul 18, 2009·Journal of Neuroscience Research·Martine UittenbogaardAnne Chiaramello
Aug 8, 2006·Gynecologic Oncology·Dong Choon ParkDae Hoon Kim
Oct 29, 2005·International Journal of Radiation Oncology, Biology, Physics·Carolyn CaoBo Lu
Apr 12, 2005·European Journal of Cell Biology·Elke JanigKurt Zatloukal
Feb 23, 2013·Neurobiology of Aging·Luba M PardoPeter Heutink
Jul 22, 2006·Biochimica Et Biophysica Acta·C A F TeixeiraM G Ribeiro
May 2, 2016·Experimental Eye Research·M Elizabeth FiniMark R Wilson
Nov 9, 2016·Frontiers in Physiology·Jiří Kanta
Jan 21, 2017·Journal of Liposome Research·Diana Guzman-VillanuevaVolkmar Weissig
Aug 5, 2009·Brain Research Reviews·Tapio NuutinenAntero Salminen
Jul 28, 2017·IUBMB Life·Dipayan Bose, Abhijit Chakrabarti
Nov 18, 2003·International Journal of Cancer. Journal International Du Cancer·Maurizio ScaltritiSaverio Bettuzzi
Oct 4, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Arpornrad SaewuNongnuj Tanphaichitr
Jul 30, 2014·Journal of the American Society of Nephrology : JASN·Poonam SansanwalMinnie M Sarwal
Jun 14, 2019·Chemical Society Reviews·Michael C OwenBirgit Strodel
Jan 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Martínez-BujidosJosé Luis Sánchez-Quesada

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