Clusterin (SGP-2) induction in rat astroglial cells exposed to prion protein fragment 106-126

The European Journal of Neuroscience
R ChiesaG Forloni

Abstract

Prion-related encephalopathies are characterized by the accumulation of an abnormal prion protein isoform (PrPSc) associated with neuronal degeneration and astrogliosis. The synthetic peptide homologous to PrP fragment 106-126 (PrP 106-126) induced in vitro neuronal apoptosis and glial proliferation. We used Northern blot analysis and the RNA polymerase chain reaction to assess the expression of several genes associated with programmed cell death and proliferation. Blots of total RNA extracted from neuronal and astroglial cells exposed to PrP 106-126 for between 1 h and 7 days were hybridized with probes recognizing c-fos, c-jun, c-myc, p53, hsp-70 and bcl-2 mRNA. Except for a slight decrease in bcl-2 mRNA in neuronal cells, no change in other transcripts was evident. Since clusterin (apolipoprotein J) mRNA levels are increased in prion-related encephalopathies and clusterin immunoreactivity has been located in association with PrPSc in Gerstmann-Sträussler-Scheinker brain, the expression of clusterin was determined in neuronal and astroglial cells chronically exposed to PrP 106-126. Although the induction of clusterin has been involved in the apoptotic mechanism in other experimental conditions, its expression was unchanged in...Continue Reading

References

Sep 1, 1992·Brain Research. Molecular Brain Research·P C MayJ A Clemens
Nov 11, 1992·Journal of Neurobiology·D P MartinE M Johnson
May 8, 1992·Brain Research·P L McGeerD G Walker
Apr 3, 1992·Cell·G I EvanD C Hancock
Apr 1, 1992·Trends in Biochemical Sciences·D E Jenne, J Tschopp
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M DanikM Suh
Mar 29, 1991·Biochemical and Biophysical Research Communications·S BettuzziC Franceschi
Aug 1, 1989·Molecular and Cellular Biology·R ButtyanI S Sawczuk
Nov 1, 1989·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·P C MayC E Finch
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J R DuguidW W Tourtellotte
Aug 25, 1987·Nucleic Acids Research·K HayashiK Yoneda
Aug 31, 1987·Biochemical and Biophysical Research Communications·J G LégerM P Tenniswood
Mar 25, 1983·Nucleic Acids Research·U NudelD Yaffe
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S A LewisN J Cowan
Dec 15, 1994·Biochemical and Biophysical Research Communications·B V ZlokovicJ Ghiso
Sep 1, 1994·The European Journal of Neuroscience·G ForloniL Verga
Jun 1, 1994·Molecular and Cellular Neurosciences·I H ZwainC Y Cheng
Sep 30, 1994·Science·H Hermeking, D Eick
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F TagliaviniG Forloni
Feb 1, 1994·The Journal of Clinical Investigation·L E FrenchJ A Schifferli
Aug 8, 1993·The Journal of Comparative Neurology·M DanikR Quirion
Oct 21, 1993·International Journal of Cancer. Journal International Du Cancer·R HuovinenY Collan
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses
Apr 8, 1993·Nature·G ForloniF Tagliavini

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Citations

May 1, 1996·Molecular and Chemical Neuropathology·G ForloniM Salmona
Jul 6, 2004·Acta Neuropathologica·M FreixesI Ferrer
Apr 21, 1999·Brain Research. Brain Research Reviews·M WaltonM Dragunow
Jun 6, 2000·Brain Research. Molecular Brain Research·I Ferrer, R Blanco
May 24, 2012·Brain Research Bulletin·Yves CharnayPanteleimon Giannakopoulos
Jan 18, 2012·Journal of Molecular Biology·Amy R WyattMark R Wilson
May 18, 2005·Journal of Molecular Biology·Alex D GreenwoodChristine Leib-Mösch
Dec 4, 2012·The International Journal of Biochemistry & Cell Biology·Sang-Wook KangDong-Hou Kim
Jan 13, 2019·Neuropathology and Applied Neurobiology·G ForloniF Tagliavini
Apr 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Justin J YerburyMark R Wilson
Jul 17, 1998·Neurobiology of Disease·D R BrownH A Kretzschmar

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