Clustering as a Means To Control Nitrate Respiration Efficiency and Toxicity in Escherichia coli

MBio
Suzy BulotAxel Magalon

Abstract

Respiration is a fundamental process that has to optimally respond to metabolic demand and environmental changes. We previously showed that nitrate respiration, crucial for gut colonization by enterobacteria, is controlled by polar clustering of the nitrate reductase increasing the electron flux through the complex. Here, we show that the formate dehydrogenase electron-donating complex, FdnGHI, also clusters at the cell poles under nitrate-respiring conditions. Its proximity to the nitrate reductase complex was confirmed by its identification in the interactome of the latter, which appears to be specific to the nitrate-respiring condition. Interestingly, we have identified a multiprotein complex dedicated to handle nitric oxide resulting from the enhanced activity of the electron transport chain terminated by nitrate reductase. We demonstrated that the cytoplasmic NADH-dependent nitrite reductase NirBD and the hybrid cluster protein Hcp are key contributors to regulation of the nitric oxide level during nitrate respiration. Thus, gathering of actors involved in respiration and NO homeostasis seems to be critical to balancing maximization of electron flux and the resulting toxicity.IMPORTANCE Most bacteria rely on the redox acti...Continue Reading

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Citations

Jul 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Sophie Saphia AbbyFabien Pierrel

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Datasets Mentioned

BETA
PXD012320

Methods Mentioned

BETA
Fluorescence
immunoprecipitation
PCR
acetylation

Software Mentioned

NarGHI
Perseus
Andromeda
MaxQuant
MicrobeJ
FIJI

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