Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants

Nature Plants
Erik JonssonGohta Goshima

Abstract

The molecular motors kinesin and dynein drive bidirectional motility along microtubules (MTs) in most eukaryotic cells. Land plants, however, are a notable exception, because they contain a large number of kinesins but lack cytoplasmic dynein, the foremost processive retrograde transporter. It remains unclear how plants achieve retrograde cargo transport without dynein. Here, we have analysed the motility of the six members of minus-end-directed kinesin-14 motors in the moss Physcomitrella patens and found that none are processive as native dimers. However, when artificially clustered into as little as dimer of dimers, the type-VI kinesin-14 (a homologue of Arabidopsis KCBP (kinesin-like calmodulin binding protein)) exhibited highly processive and fast motility (up to 0.6 μm s(-1)). Multiple kin14-VI dimers attached to liposomes also induced transport of this membrane cargo over several microns. Consistent with these results, in vivo observations of green fluorescent protein-tagged kin14-VI in moss cells revealed fluorescent punctae that moved processively towards the minus-ends of the cytoplasmic MTs. These data suggest that clustering of a kinesin-14 motor serves as a dynein-independent mechanism for retrograde transport in p...Continue Reading

References

Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·H SongS A Endow
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·D G OppenheimerM D Marks
Jan 10, 1998·The Journal of Biological Chemistry·E Pechatnikova, E W Taylor
Nov 24, 1999·Nature Cell Biology·S A Endow
May 15, 2001·Traffic·C J LawrenceR K Dawe
Sep 28, 2002·Science·Michio TomishigeRonald D Vale
Mar 24, 2004·Current Opinion in Cell Biology·Teruo Shimmen, Etsuo Yokota
Jun 17, 2005·Molecular Biology of the Cell·Gohta Goshima, Ronald D Vale
Nov 16, 2005·Annual Review of Genetics·David Cove
Dec 31, 2005·Nature·Nicholas F EndresRonald D Vale
Mar 21, 2007·Nature Methods·Maximilian H Ulbrich, Ehud Y Isacoff
Oct 9, 2007·Biophysical Journal·Colin Echeverría AitkenJoseph D Puglisi
Dec 7, 2007·Nature·Peter BielingThomas Surrey
Sep 24, 2009·Nature Reviews. Molecular Cell Biology·Nobutaka HirokawaShinsuke Niwa
Sep 30, 2009·The Journal of Cell Biology·Sivaraj Sivaramakrishnan, James A Spudich
Oct 24, 2009·Nature Reviews. Molecular Cell Biology·Kristen J Verhey, Jennetta W Hammond
Apr 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki SuetsuguMasamitsu Wada
Dec 8, 2010·The Journal of Cell Biology·Yuk-Kwan ChoiRobert Z Qi
Jul 28, 2012·Frontiers in Plant Science·Giampiero Cai, Mauro Cresti
Oct 23, 2012·Frontiers in Plant Science·Zhiyuan ShenLuis Vidali
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ken'ya FurutaHiroaki Kojima
May 29, 2013·The Journal of General Physiology·Keegan E Hines
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tomohiro MikiGohta Goshima
Jul 19, 2014·Science·Richard J McKenneyRonald D Vale
Aug 15, 2014·Nature Reviews. Molecular Cell Biology·William O Hancock

❮ Previous
Next ❯

Citations

Nov 12, 2015·Current Opinion in Plant Biology·Yuh-Ru Julie LeeBo Liu
Sep 24, 2015·Current Biology : CB·Sabine Müller
Jan 1, 2015·Nature Plants·Ram Dixit
Apr 17, 2016·Journal of Hepatology·Marie-Lise BlondotMichael Kann
Aug 2, 2016·Trends in Plant Science·Henrik Buschmann, Sabine Zachgo
Oct 5, 2016·Current Opinion in Cell Biology·Divya Pathak, Roop Mallik
Jan 27, 2017·Biology·Moé Yamada, Gohta Goshima
Jun 21, 2017·Current Biology : CB·Stephen R Norris, Ryoma Ohi
Jan 21, 2018·The Journal of Cell Biology·Yang YueKristen J Verhey
Nov 25, 2017·Journal of Plant Research·Yoshihisa Oda
Mar 1, 2018·Annual Review of Plant Biology·Andreas Nebenführ, Ram Dixit
May 14, 2018·FEBS Letters·Allison M GickingWeihong Qiu
Nov 2, 2018·Biophysics Reviews·Shu-Zon WuMagdalena Bezanilla
Feb 28, 2018·Scientific Reports·Xiaolu XuPeter Nick
Jan 5, 2017·Nature Communications·Andrew R PopchockWeihong Qiu
Apr 27, 2017·The Journal of Cell Biology·Moé YamadaGohta Goshima
Jan 18, 2020·Journal of Experimental Botany·Sergio Galindo-TrigoLisa M Smith
Aug 28, 2020·Cell Adhesion & Migration·Iftikhar Ali, Wei-Cai Yang
Jul 10, 2018·Nature Communications·Stephen R NorrisRyoma Ohi
Jul 6, 2017·Scientific Reports·Josie L HilleyJohn E Mullet
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Susan H BrawleySimon E Prochnik
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Kristin I SchimertKristen J Verhey
Jun 17, 2020·Journal of Cell Science·Marcus BraunZdenek Lansky
Apr 25, 2020·The Journal of Cell Biology·Wilco NijenhuisLukas C Kapitein
Jan 23, 2021·Nature Materials·Sarah TriclinManuel Théry
Jul 19, 2018·Current Biology : CB·Pan WangWeihong Qiu
Apr 3, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Holly V GoodsonSusan H Brawley
May 7, 2021·Annual Review of Biophysics·John T CantyAhmet Yildiz
Aug 1, 2020·Current Biology : CB·Kuo-Fu TsengWeihong Qiu

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