Clustering of South African Helicobacter pylori isolates from peptic ulcer disease patients is demonstrated by repetitive extragenic palindromic-PCR fingerprinting

Journal of Clinical Microbiology
M KiddJ A Louw

Abstract

The present report assesses the association between clonal groupings, disease, and the virulence fingerprint of 76 South African Helicobacter pylori cagA(+) strains isolated from 57 Cape-colored subjects. Two methods, repetitive extragenic palindromic (REP)-PCR and random amplified polymorphic DNA (RAPD)-PCR, were used to generate DNA fingerprints, and computer-assisted analysis was used to derive clusters. The two PCR techniques were only partially complementary (48%). REP-PCR fingerprints identified a distinct pathological cluster consisting of strains from 63% of the patients and was strongly associated with both disease (P < 0.00001) and the vacuolating cytotoxin A (vacA) signal sequence type (P < 0.003). RAPD-PCR fingerprinting was not associated with disease and was less strongly associated with vacA (P < 0.05) than REP-PCR was. Hierarchical analysis indicated that isolates from patients with peptic ulcer disease tended to cluster differently than isolates from patients with gastritis alone or gastric adenocarcinoma. These relationships are consistent with a loosely clonal population structure associated with disease for H. pylori in the Cape-colored population in South Africa.

References

Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·S SuerbaumM Achtman

❮ Previous
Next ❯

Citations

Jan 5, 2002·Journal of Clinical Microbiology·Sean O HynesUNKNOWN Eurohepygast Study Group
Mar 23, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Alba Edith VegaHumberto Jesús Silva
Apr 28, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Sugimoto, Y Yamaoka
Apr 5, 2017·World Journal of Gastroenterology : WJG·Javier Andres Bustamante-RengifoLuis Eduardo Bravo
Oct 27, 2007·Arquivos De Gastroenterologia·Anita P O GodoyJosé Pedrazzoli
Jan 11, 2003·Environmental Science & Technology·Joyce M SimpsonDonald J Reasoner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.