Clustering Small Dendrimers into Nanoaggregates for Efficient DNA and siRNA Delivery with Minimal Toxicity

Advanced Healthcare Materials
Chongyi LiuYiyun Cheng

Abstract

Cationic dendrimers are widely used as nonviral gene vectors, however, current gene materials based on dendrimers are either little effective or too toxic on the transfected cells. Here, a facile strategy is presented to prepare high efficient dendrimers with low transfection toxicity. Small dendrimers with 2 nm are clustered into nanoaggregates (≈100 nm) via phenylboronic acid modification and the self-assembled materials enable efficient DNA and siRNA delivery on several cell lines. The clustered nanostructures can disassemble into small dendrimers in acidic conditions thus exerting significantly less toxicity on the transfected cells. Further structure-function relationship studies reveal that both the phenyl group and boronic acid group play essential roles in the self-assembly and gene delivery processes. The transfection efficacy of phenylboronic acid-modified dendrimers can be down-regulated by blocking the boronic acid groups on dendrimers with diols or degrading the groups with hydrogen peroxide. This study provides a facile strategy in the development of efficient and biocompatible gene vectors based on low molecular weight polymers and clearly demonstrates the structure-function relationship of phenylboronic acid-mod...Continue Reading

References

Jul 30, 2005·Nature Reviews. Drug Discovery·Daniel W PackPatrick S Stayton
Nov 25, 2005·Advanced Drug Delivery Reviews·Sönke Svenson, Donald A Tomalia
Nov 29, 2005·Advanced Drug Delivery Reviews·Christine DufèsAndreas G Schätzlein
Dec 8, 2005·Nature Biotechnology·Cameron C LeeFrancis C Szoka
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Miriam BreunigAchim Goepferich
Jul 2, 2009·Methods in Molecular Biology·Jean-Pierre GilletChava Kimchi-Sarfaty
Feb 9, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·José L SantosHelena Tomás
Jul 14, 2010·Chemical Communications : Chem Comm·Qi PengRenxi Zhuo
Dec 2, 2010·Organic & Biomolecular Chemistry·Julia Morales-SanfrutosFrancisco Santoyo-Gonzalez
Dec 6, 2011·Nature Materials·Jiangbing ZhouW Mark Saltzman
Dec 16, 2011·Nature Materials·Enrico Mastrobattista, Wim E Hennink
Apr 21, 2012·Macromolecular Bioscience·Fujie ChenRenxi Zhuo
Aug 22, 2012·Angewandte Chemie·Mitsuru NaitoKazunori Kataoka
Oct 12, 2012·Journal of the American Chemical Society·Hongmei LiuYiyun Cheng
Mar 19, 2013·Journal of the American Chemical Society·Hanxiang ZengZhibin Guan
Jun 28, 2013·Journal of the American Chemical Society·Johannes F TeichertJeffrey W Bode
Jul 19, 2013·Chemical Society Reviews·Ying Guan, Yongjun Zhang
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jian YangShibin Zhou
Sep 14, 2013·Journal of the American Chemical Society·Stephanie DeshayesKazunori Kataoka
Oct 24, 2013·Nature Materials·Rosemary KanastyDaniel Anderson
Oct 31, 2013·Journal of the American Chemical Society·Kien Voon KongMalini Olivo
Jul 30, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Katsuhiko SatoJun-ichi Anzai
Jan 30, 2015·Macromolecular Rapid Communications·Hao MengTao Chen
May 7, 2015·Chemical Reviews·Jiepin YangYiyun Cheng

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Citations

Jan 20, 2016·Bioconjugate Chemistry·Mingming Wang, Yiyun Cheng
May 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Zibiao LiYun-Long Wu
Aug 23, 2017·Organic & Biomolecular Chemistry·Carlos E Gutierrez-UlloaRafael Gómez
Feb 14, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Long ZhangXingjie Zan
Oct 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wanwan ShenYiyun Cheng
Nov 22, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Xuan LiuWei Huang
Feb 12, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu-Lin SuShang-Hsiu Hu
Mar 10, 2021·Chemical Society Reviews·Jia Lv, Yiyun Cheng

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