Clusters of nucleosomes containing chromosomal protein HMG-17 in chromatin

Journal of Molecular Biology
Y V PostnikovM Bustin

Abstract

Chromosomal proteins HMG-14 and HMG-17 are nucleosome binding proteins which can function as architectural elements to alter the structure of the chromatin fiber and enhance transcription from chromatin templates. Here we study the spatial organization of these HMG proteins in the nucleus and the distribution of nucleosomes containing HMG-17 in the chromatin fiber. By confocal immunofluorescence microscopy we find that HMG-14/17 proteins are clustered into foci containing either HMG-14 or HMG-17. These results suggest that HMG-14/17 proteins segregate into distinct nuclear domains. Indeed, immunofractionation of defined length oligonucleosomes, with affinity pure antibodies to HMG-17, indicates that oligonucleosomes containing HMG-17 are devoid of HMG-14. Quantitative analysis indicates that in cellular chromatin nucleosomes containing HMG-17 are clustered. The average size of the cluster is six contiguous HMG-17-containing nucleosomes. The nucleosomes in this cluster contain either two or zero molecules of HMG-17 and a complete set of four core histones. We suggest that HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber, to unfold the higher order chromatin structure and facilitate access to th...Continue Reading

Citations

Jan 12, 1999·The Biochemical Journal·M KratzmeierD Doenecke
Feb 22, 2008·Molecular Biology of the Cell·Srujana CherukuriMichael Bustin
Aug 2, 2002·EMBO Reports·Frédéric CatezMichael Bustin
Feb 1, 2011·Nucleic Acids Research·Mark RochmanMichael Bustin
Jul 5, 2001·Molecular and Cellular Biology·M Prymakowska-BosakM Bustin
May 4, 2004·Molecular and Cellular Biology·Frédéric CatezMichael Bustin
Apr 15, 2004·Molecular and Cellular Biology·Katherine L WestMichael Bustin
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Frédéric CatezMichael Bustin
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gabi GerlitzMichael Bustin
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·L TrieschmannM Bustin
Dec 29, 2010·Journal of Nucleic Acids·Michelle S OngCurt A Davey
Oct 5, 2014·Molecular Biology and Evolution·Rodrigo González-RomeroJuan Ausió
Oct 2, 2007·Progress in Retinal and Eye Research·Ales Cvekl, Melinda K Duncan
Nov 22, 2001·Journal of Endotoxin Research·C J CzuraK J Tracey
Feb 6, 2018·Journal of Immunotherapy·Jiao ChenPing Zhang
Oct 6, 2005·Journal of Leukocyte Biology·Yun FengBoyao Wang
Jan 14, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Vassiliki A Boumba, Konstantin Seferiadis
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Hardi LührsThomas Menzel
Sep 7, 2000·The Journal of Biological Chemistry·H ShirakawaY Postnikov
Oct 28, 2004·Biochemical Society Transactions·K L West
Jul 7, 2001·Trends in Biochemical Sciences·M Bustin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.