CML10, a variant of calmodulin, modulates ascorbic acid synthesis

The New Phytologist
Kwang-Moon ChoSung Han Ok

Abstract

Calmodulins (CaMs) regulate numerous Ca(2+) -mediated cellular processes in plants by interacting with their respective downstream effectors. Due to the limited number of CaMs, other calcium sensors modulate the regulation of Ca(2+) -mediated cellular processes that are not managed by CaMs. Of 50 CaM-like (CML) proteins identified in Arabidopsis thaliana, we characterized the function of CML10. Yeast two-hybrid screening revealed phosphomannomutase (PMM) as a putative interaction partner of CML10. In vitro and in vivo interaction assays were performed to analyze the interaction mechanisms of CML10 and PMM. PMM activity and the phenotypes of cml10 knock-down mutants were studied to elucidate the role(s) of the CML10-PMM interaction. PMM interacted specifically with CML10 in the presence of Ca(2+) through its multiple interaction motifs. This interaction promoted the activity of PMM. The phenotypes of cml10 knock-down mutants were more sensitive to stress conditions than wild-type plants, corresponding with the fact that PMM is an enzyme which modulates the biosynthesis of ascorbic acid, an antioxidant. The results of this research demonstrate that a calcium sensor, CML10, which is an evolutionary variant of CaM, modulates the st...Continue Reading

References

Jan 1, 1986·Radiation and Environmental Biophysics·H K LichtenthalerG Schmuck
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·T JabsD Scheel
Jun 10, 1998·Nature·G L WheelerN Smirnoff
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KovtunJ Sheen
Oct 3, 2000·The EMBO Journal·A DelaunayM B Toledano
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Nicholas SmirnoffFrank A Loewus
May 30, 2001·Trends in Plant Science·H Knight, M R Knight
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·T Yang, B W Poovaiah
May 9, 2002·Journal of Experimental Botany·Eva VranováFrank Van Breusegem
Jun 5, 2002·The Plant Cell·Dale SandersJeffrey F Harper
Jun 14, 2003·Plant Physiology·Estelle M HrabakAlice C Harmon
Jul 3, 2003·Journal of Structural and Functional Genomics·K L YapM Ikura
Oct 15, 2003·Trends in Plant Science·Tianbao Yang, B W Poovaiah
May 19, 2004·FEBS Letters·Arthur KerschenAndreas E Müller
Jul 13, 2004·Plant Physiology·Maike C Rentel, Marc R Knight
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
May 3, 2005·Annual Review of Plant Biology·Nicolas BouchéHillel Fromm
Jul 19, 2005·Trends in Plant Science·Elizabeth McCormackJanet Braam
Oct 26, 2005·Plant Physiology·Valeria PavetChristine H Foyer
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
May 9, 2006·Journal of Molecular Biology·Zenon Grabarek
Jan 23, 2008·The New Phytologist·Wing-Yen Francisca LiHon-Ming Lam
Mar 19, 2008·Physiologia Plantarum·Gad MillerRon Mittler
Jan 6, 2011·Plant Physiology·Christine H Foyer, Graham Noctor
May 7, 2013·International Journal of Molecular Sciences·Mirza HasanuzzamanMasayuki Fujita

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Citations

Apr 29, 2016·Protein Science : a Publication of the Protein Society·Rosario ValloneAlessandra Astegno
Nov 12, 2016·Plant & Cell Physiology·Xiaoyang ZhuJean-Philippe Galaud
May 2, 2018·International Journal of Molecular Sciences·Valentina La VerdeAlessandra Astegno
May 5, 2017·Plant Signaling & Behavior·Xiaoyang ZhuJean-Philippe Galaud
Mar 11, 2020·International Journal of Molecular Sciences·Ronan C BroadAlexander A T Johnson
Jul 27, 2017·Frontiers in Chemistry·Ifigeneia Mellidou, Angelos K Kanellis
Feb 9, 2019·Frontiers in Plant Science·Mario FenechMiguel A Botella
Aug 17, 2019·Journal of Inorganic Biochemistry·Matteo TrandeAlessandra Astegno
Jul 13, 2021·Frontiers in Plant Science·Heike WolffMartin Hülskamp
Sep 17, 2021·The Plant Journal : for Cell and Molecular Biology·Qiguo SunZhenfei Guo

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