CMV promotor activity during ES cell differentiation: potential insight into embryonic stem cell differentiation

Cell Biology International
Bandita BagchiShyamala Mani

Abstract

The activity of the P(CMV IE) promoter was studied during the differentiation of ES cells into neurons. In order to do this, stable embryonic stem (ES) cell lines that express enhanced green fluorescent protein (EGFP) under the control of P(CMV IE) were created and these ES cells were differentiated by aggregation of cells in the presence of retinoic acid (RA). Based on our observations that the activity of P(CMV IE) was highest in undifferentiated cells, and that cell-cell interaction and addition of RA that lead to enhanced cell proliferation also increased expression from P(CMV IE), we hypothesized that the activity of P(CMV IE) was positively regulated in cycling cells. However, when analysis was done at the single cell level it was found that BrdU label and EGFP expression were not correlated. EGFP expression was found to be down-regulated in many cells that were BrdU positive and conversely there were significant numbers of BrdU negative cells that were EGFP positive. Further, P(CMV IE) activity was not observed in cells that were nestin positive or in differentiated neurons, but P(CMV IE) was active in cells with a fibroblast-like morphology. Finally, several proteins present in undifferentiated ES cells were found to bi...Continue Reading

Citations

May 2, 2008·Stem Cells and Development·Rong WangHuang-Tian Yang
Jan 2, 2014·Stem Cells and Development·Naohisa WadaAkifumi Akamine
Mar 1, 2012·Differentiation; Research in Biological Diversity·Bruno Daniele LeoniArmando Felsani
Mar 14, 2009·Differentiation; Research in Biological Diversity·Shailesh Kumar GuptaShyamala Mani
Dec 9, 2008·Journal of Neuroscience Methods·Maja-Theresa DieterlenJohannes Schwarz
Sep 20, 2008·Journal of Neuroscience Methods·Cristina M BäckmanAndreas C Tomac
Nov 11, 2009·Acta Biochimica Et Biophysica Sinica·Xiaowen GongXuejun Zhang
Apr 3, 2020·Molecular Biology Reports·Felix StengelPhilipp Capetian

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