CNS neuronal focal adhesion kinase forms clusters that co-localize with vinculin

Journal of Neuroscience Research
G R StevensW L Klein

Abstract

Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that appears to play a central role in integrin-mediated signal transduction in non-neuronal cells, linking the extracellular matrix to the actin-based cytoskeleton at focal adhesion contacts. Biochemical analysis has revealed the presence of FAK immunoreactivity in cells of neuronal lineage (Zhang et al., 1994) and in the CNS (Burgaya et al. 1995; Grant et al., 1995). In the current work, we have examined the immunodistribution of FAK in nerve cell cultures and tissue sections from the rat CNS. Cultures of B103 CNS neuroblastoma cells and primary cultures of hippocampal neurons both showed abundant FAK immunoreactivity in nerve cell bodies. Immunoreactivity also extended into neurites and growth cones. The most striking feature of FAK distribution was the presence of short, punctate clusters of high FAK concentration. These FAK clusters were maintained in triton-extracted cell ghosts, indicating association with the cytoskeleton. Double-label confocal imaging showed that clusters of FAK coincided with clusters of vinculin, another actin-associated signal transduction molecule implicated in control of growth cone motility. Data from hippocampal sections verif...Continue Reading

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Citations

May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P LambertW L Klein
Jul 30, 2011·PloS One·Matthew A HemphillKevin Kit Parker
Nov 14, 1997·The Journal of Biological Chemistry·F BurgayaJ A Girault
May 23, 2013·Cell Communication and Signaling : CCS·Matthew P KeaseyTheo Hagg
Oct 3, 1999·The Journal of Biological Chemistry·J FujimotoT Yamamoto
Jun 18, 2002·Journal of Neurochemistry·Lucilla Bongiorno-BorboneFabio Benfenati
Jul 12, 2008·Annals of Biomedical Engineering·Frank Xue JiangNoshir A Langrana
Feb 6, 1998·Journal of Neuroscience Research·M M BergW L Klein
Mar 21, 2000·Journal of Neuroscience Research·A RenaudinL McKerracher
Feb 23, 2000·Molecular Cell Biology Research Communications : MCBRC·D O Clegg
Jun 21, 2021·Cellular and Molecular Life Sciences : CMLS·Pranay MandalNarendrakumar Ramanan
Dec 7, 2007·Experimental Cell Research·Ines LahmannHans-Henning Arnold
Sep 25, 1997·Journal of Medicinal Chemistry·J L LauerG B Fields

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