Co- and posttranslational modification of the alpha(1B)-adrenergic receptor: effects on receptor expression and function

Biochemistry
Katja BjörklöfS Cotecchia

Abstract

We have characterized the maturation, co- and posttranslational modifications, and functional properties of the alpha(1B)-adrenergic receptor (AR) expressed in different mammalian cells transfected using conventional approaches or the Semliki Forest virus system. We found that the alpha(1B)-AR undergoes N-linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation. Pulse-chase labeling experiments in BHK-21 cells demonstrate that the alpha(1B)-AR is synthesized as a 70 kDa core glycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half-time of approximately 2 h. N-Linked glycosylation of the alpha(1B)-AR occurs at four asparagines on the N-terminus of the receptor. Mutations of the N-linked glycosylation sites did not have a significant effect on receptor function or expression. Surprisingly, receptor mutants lacking N-linked glycosylation migrated as heterogeneous bands in SDS-PAGE. Our findings demonstrate that N-linked glycosylation and phosphorylation, but not palmitoylation or O-linked glycosylation, contribute to the structural heterogeneity of the alpha(1B)-AR as it is observed in SDS-PAGE. The modifications found ...Continue Reading

References

Aug 1, 1995·Quarterly Reviews of Biophysics·R Grisshammer, C G Tate
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·K LundstromP Liljestrom

❮ Previous
Next ❯

Citations

Feb 20, 2003·European Journal of Pharmacology·Andre S PupoKenneth P Minneman
Jul 6, 2006·Trends in Biotechnology·Samuel WagnerJan-Willem de Gier
Dec 31, 2002·Journal of Receptor and Signal Transduction Research·Susanna CotecchiaFrancesca Fanelli
Feb 15, 2014·Journal of Biomolecular Screening·John W Cuozzo, Holly H Soutter
Mar 20, 2003·The Journal of Biological Chemistry·Dario DivianiSusanna Cotecchia
Dec 15, 2004·Journal of Pharmacological and Toxicological Methods·K H S ArunP Ramarao
Jan 27, 2009·Biochemical and Biophysical Research Communications·Takami Yurugi-KobayashiTakuya Kobayashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.