Co-administration of polyphosphazenes with CpG oligodeoxynucleotides strongly enhances immune responses in mice immunized with Hepatitis B virus surface antigen

Vaccine
G MutwiriL A Babiuk

Abstract

An emerging paradigm in vaccinology is that multiple adjuvant combinations may be more effective than individual adjuvants in enhancing immune responses to vaccine antigens. We investigated whether the polyphosphazenes used in combination with CpG oligodeoxynucleotides (ODN) were potent adjuvant formulations. BALB/c mice were immunized subcutaneously with Hepatitis B surface antigen (HBsAg) alone, or in various combinations with poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP), poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) or CpG ODN. All three adjuvants enhanced HBsAg-specific IgG1 antibody responses with PCEP inducing the highest responses. PCEP and CpG ODN significantly enhanced the Th1-associated antibody isotype IgG2a. As expected CpG ODN induced predominantly Th1-type immune responses while PCEP was associated with mixed Th1/Th2 immune responses. Interestingly, PCEP and PCPP synergized with CpG ODN to further enhance antibody responses. Since the mechanisms which mediate the adjuvant activity of polyphosphazenes are not fully understood, we investigated whether PCEP and PCPP could stimulate innate immune responses. Incubation of mouse splenocytes with PCEP or PCPP (in the absence of antigen) stimulated p...Continue Reading

References

Jan 1, 1995·International Journal of Clinical & Laboratory Research·A W Heath
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·D M KlinmanA M Krieg
Aug 23, 2001·Advanced Drug Delivery Reviews·P N Boyaka, J R McGhee
Sep 22, 2001·Biomolecular Engineering·D T O'HaganM Singh
Feb 4, 2003·Nature Reviews. Immunology·Giorgio Trinchieri
Sep 14, 2004·Biomacromolecules·Alexander K AndrianovMark P LeGolvan
Aug 6, 2005·Vaccine·Risini D WeeratnaHeather L Davis

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Citations

Sep 9, 2010·Expert Review of Vaccines·Moses O OyewumiZhengrong Cui
Dec 18, 2010·Expert Review of Vaccines·George MutwiriAndrew Potter
Dec 25, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Xinran LiZhengrong Cui
Jan 18, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ying-Ju LinFuu-Jen Tsai
Jan 27, 2009·Advanced Drug Delivery Reviews·Padma MalyalaManmohan Singh
Jan 27, 2009·Advanced Drug Delivery Reviews·Aliasger K Salem, George J Weiner
Jan 24, 2009·Advanced Drug Delivery Reviews·George MutwiriLorne A Babiuk
May 4, 2011·Journal of Applied Toxicology : JAT·Li LiuBo Li
Jun 12, 2012·Vaccine·Alexander K Andrianov, George Mutwiri
Apr 24, 2012·Molecular Immunology·Sunita AwateGeorge Mutwiri
Aug 26, 2010·Journal of Immune Based Therapies and Vaccines·Nelson F EngGeorge K Mutwiri
Feb 5, 2015·Clinical and Experimental Vaccine Research·Bradford S PowellPeter C Fusco
Nov 23, 2012·Journal of the Royal Society, Interface·Akhilesh Kumar Shakya, Kutty Selva Nandakumar
Oct 9, 2018·Cell and Tissue Research·Royford MagiriHeather L Wilson
Jul 1, 2012·Emerging Microbes & Infections·Lorne A Babiuk, Volker Gerdts
Dec 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexander K Andrianov, Robert Langer
Mar 26, 2021·Frontiers in Bioengineering and Biotechnology·Dylan J ChandGeorge K Mutwiri

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