Co-crystal Structure of Thermosynechococcus elongatus Sucrose Phosphate Synthase With UDP and Sucrose-6-Phosphate Provides Insight Into Its Mechanism of Action Involving an Oxocarbenium Ion and the Glycosidic Bond

Frontiers in Microbiology
Yuying LiJiyong Su

Abstract

In green species, sucrose can help antagonize abiotic stress. Sucrose phosphate synthase (SPS) is a well-known rate-limiting enzyme in the synthesis of sucrose. To date, however, there is no known crystal structure of SPS from plant or cyanobacteria. In this study, we report the first co-crystal structure of SPS from Thermosynechococcus elongatus with UDP and sucrose-6-phosphate (S6P). Within the catalytic site, the side chains of His158 and Glu331, along with two phosphate groups from UDP, form hydrogen bonds with the four hydroxyl groups of the glucose moiety in S6P. This association causes these four hydroxyl groups to become partially negatively charged, thus promoting formation of the C1 oxocarbenium ion. Breakage of the hydrogen bond between His158 and one of the hydroxyl groups may trigger covalent bond formation between the C1 oxocarbenium ion and the C2 hydroxyl of fructose-6-phosphate. Consistent with our structural model, we observed that two SPS mutants, H158A and E331A, lost all catalytic activity. Moreover, electron density of residues from two loops (loop1 and loop2) in the SPS A-domain was not observed, suggest their dynamic nature. B-factor analysis and molecular dynamics stimulations of the full-length enzyme ...Continue Reading

References

May 1, 1992·The Biochemical Journal·J L Huber, S C Huber
Nov 1, 1990·Archives of Biochemistry and Biophysics·S C Huber, J L Huber
May 1, 1989·Archives of Biochemistry and Biophysics·J L HuberT H Nielsen
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·D ToroserS C Huber
Aug 5, 2000·The Journal of Biological Chemistry·E CidJ C Ferrer
Sep 26, 2000·The Journal of Biological Chemistry·P L AbdianR A Geremia
Sep 27, 2000·Critical Reviews in Biochemistry and Molecular Biology·H Winter, S C Huber
Apr 16, 2002·Plant Physiology·John Edward Lunn
Sep 21, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yasukazu NakamuraSatoshi Tabata
Feb 25, 2003·Trends in Plant Science·Graciela L Salerno, Leonardo Curatti
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Igor N Berezovsky, Eugene I Shakhnovich
Mar 1, 1991·Applied and Environmental Microbiology·N HershkovitzY Cohen
May 1, 1982·Plant Physiology·J Amir, J Preiss
Apr 25, 2007·Nucleic Acids Research·Ian W DavisDavid C Richardson
Apr 9, 2008·The Journal of Biological Chemistry·Matthew W VettingJohn S Blanchard
Jun 4, 2008·Annual Review of Biochemistry·L L LairsonS G Withers
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
May 28, 2010·BMC Structural Biology·Todd J Taylor, Iosif I Vaisman
Aug 11, 2010·Phytochemistry·Julia WindJohannes Hanson
Aug 9, 2011·Nature Chemical Biology·Seung Seo LeeBenjamin G Davis
Nov 15, 2012·Accounts of Chemical Research·Neal J Zondlo
Apr 27, 2013·FEBS Letters·Giselle M A Martínez-NoëlGraciela L Salerno
Jun 1, 2013·Metabolic Engineering·Wei DuXuefeng Lu
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
glycotransferase
glycotransfereases
enzymatic assay
chemical modification

Software Mentioned

GROMACS
CCP4
Phenix
CHARMM36
Phaser
xmgrace
CGenFF
MolProbity
XDS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.