Co-crystallization and characterization of the photosynthetic reaction center-cytochrome c2 complex from Rhodobacter sphaeroides

Biochemistry
N AdirG Feher

Abstract

The photosynthetic reaction center (RC) of Rhodobacter sphaeroides and cytochrome c2 (cyt c2), its physiological secondary electron donor, have been co-crystallized. The molar ratio of RC/cyt c2 was found by SDS-PAGE and optical absorbance changes in the co-crystals to be 4. The crystals diffracted X-rays to 3.5 angstroms. However, the resolution degraded during data collection. A data set, 82.5% complete, was collected to 4.5 angstroms. The crystals belong to the tetragonal space group P4(3)2(1)2, with unit cell dimensions of a = b = 142.7 angstroms and c = 254.8 angstroms. The positions of the RCs in the unit cell were determined by molecular replacement. A comparable search for the cyt c2 by this method was unsuccessful because of the small contribution of the cytochrome to the total scattering and because of its low occupancy. The cyt c2 was positioned manually into patches of difference electron density, adjacent to the periplasmic surface of the M polypeptide subunit of the RC. The difference electron density was not sufficient for precise positioning of the cyt c2, and its orientation was modeled by placing the exposed edge of the heme toward the primary donor of the reaction center D and by forming pairs for electrostat...Continue Reading

Citations

Sep 27, 2000·Biochimica Et Biophysica Acta·P K Fyfe, M R Jones
Mar 9, 2000·Trends in Microbiology·A R Crofts
Jul 17, 1998·Current Opinion in Chemical Biology·D Beratan, S Skourtis
Sep 1, 2002·Journal of Biological Physics·O MiyashitaJ N Onuchic
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shirley LaromNoam Adir
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MyllykallioF Daldal
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
Apr 12, 2013·Photosynthesis Research·T L OlsonJ P Allen
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Osamu MiyashitaMelvin Y Okamura
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·H L AxelrodG Feher
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Clément LieutaudBarbara Schoepp-Cothenet
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·F A TezcanH B Gray
Jul 17, 2014·Biochemistry·Oleksandr KokhanDavid M Tiede
Nov 18, 2005·Biochimica Et Biophysica Acta·Ran FriedmanMenachem Gutman
Nov 1, 2002·Biochimica Et Biophysica Acta·Michael R JonesRichard J Cogdell
Jun 23, 2009·Biochimica Et Biophysica Acta·Nina S PonomarenkoJames R Norris
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·George Feher
Nov 8, 2013·Molecular Based Mathematical Biology·Chuan LiEmil Alexov
Aug 4, 1999·Nature·A S Kondrashov, F A Kondrashov
Feb 19, 2021·Nature Communications·Tomoaki KawakamiZheng-Yu Wang-Otomo
Sep 26, 2009·Biochemistry·Stefano SantabarbaraFabrice Rappaport
May 2, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Olga FedotovskayaPia Ädelroth
Sep 2, 2004·Journal of the American Chemical Society·Seong A KangBrian R Crane

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.