Co-culture and biogeography of Prochlorococcus and SAR11

The ISME Journal
Jamie W BeckerSallie W Chisholm

Abstract

Prochlorococcus and SAR11 are among the smallest and most abundant organisms on Earth. With a combined global population of about 2.7 × 1028 cells, they numerically dominate bacterioplankton communities in oligotrophic ocean gyres and yet they have never been grown together in vitro. Here we describe co-cultures of Prochlorococcus and SAR11 isolates representing both high- and low-light adapted clades. We examined: (1) the influence of Prochlorococcus on the growth of SAR11 and vice-versa, (2) whether Prochlorococcus can meet specific nutrient requirements of SAR11, and (3) how co-culture dynamics vary when Prochlorococcus is grown with SAR11 compared with sympatric copiotrophic bacteria. SAR11 grew 15-70% faster in co-culture with Prochlorococcus, while the growth of the latter was unaffected. When Prochlorococcus populations entered stationary phase, this commensal relationship rapidly became amensal, as SAR11 abundances decreased dramatically. In parallel experiments with copiotrophic bacteria; however, the heterotrophic partner increased in abundance as Prochlorococcus densities leveled off. The presence of Prochlorococcus was able to meet SAR11's central requirement for organic carbon, but not reduced sulfur. Prochlorococc...Continue Reading

References

May 3, 1990·Nature·Stephen J GiovannoniK G Field
Jun 12, 1998·Nature·Lisa R MooreSallie W Chisholm
Jul 31, 2002·Applied and Environmental Microbiology·Stephanie A Connon, Stephen J Giovannoni
Aug 9, 2002·Nature·Michael S RappéStephen J Giovannoni
Dec 20, 2002·Nature·Robert M MorrisStephen J Giovannoni
Aug 20, 2005·Science·Stephen J GiovannoniEric J Mathur
Oct 28, 2006·Science·Maria Vila-CostaRonald Kiene
May 19, 2007·Science·Claudia R Benitez-NelsonEun Jin Yang
Aug 19, 2007·Trends in Microbiology·Maureen L Coleman, Sallie W Chisholm
Dec 28, 2007·PLoS Genetics·Gregory C KettlerSallie W Chisholm
Mar 14, 2008·Nature·H James TrippStephen J Giovannoni
May 27, 2008·Applied and Environmental Microbiology·J Jeffrey MorrisErik R Zinser
Jan 8, 2009·Environmental Microbiology·H James TrippStephen J Giovannoni
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·D J ScanlanF Partensky
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Federico M LauroRicardo Cavicchioli
Nov 6, 2009·Environmental Microbiology·M S SchwalbachStephen J Giovannoni
May 14, 2010·The ISME Journal·Rex R MalmstromSallie W Chisholm
Feb 18, 2011·The ISME Journal·Daniel SherSallie W Chisholm
Sep 3, 2011·PloS One·Jing SunStephen J Giovannoni
Oct 27, 2011·PLoS Biology·Eric KarsentiTara Oceans Consortium
Feb 11, 2012·Science·Stephen J Giovannoni, Kevin L Vergin
Jul 19, 2012·Molecular Systems Biology·Mark V BrownRicardo Cavicchioli
Sep 7, 2012·PloS One·Jacob R WaldbauerSallie W Chisholm
Apr 13, 2013·Antimicrobial Agents and Chemotherapy·Daniel V ZurawskiThomas J Palys
Apr 30, 2013·The Journal of Microbiology·H James Tripp
May 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pedro FlombaumAdam C Martiny
Apr 18, 2014·The ISME Journal·Stephen J GiovannoniBen Temperton
Dec 2, 2014·Nature Reviews. Microbiology·Steven J BillerSallie W Chisholm
Jan 1, 2014·Scientific Data·Steven J BillerSallie W Chisholm
May 23, 2015·Science·Shinichi SunagawaPeer Bork
Jun 2, 2015·Scientific Data·Stéphane PesantTara Oceans Consortium Coordinators
Jun 28, 2015·Molecular Biology and Evolution·Haiwei LuoAustin L Hughes
Dec 17, 2015·Genome Biology·Luisa W HugerthAnders F Andersson
Apr 14, 2016·Nature Communications·Peter MenzelAnders Krogh
Jun 4, 2016·The ISME Journal·Steven J BillerSallie W Chisholm
Jul 6, 2016·Scientific Data·Mohamed F HaroonUlrich Stingl
Oct 1, 2016·Annual Review of Marine Science·Stephen J Giovannoni
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rogier BraakmanSallie W Chisholm
Jun 13, 2017·Nature Biotechnology·Supratim MukherjeeNikos C Kyrpides
Jun 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Andres Cubillos-RuizSallie W Chisholm
Sep 13, 2017·Nature Microbiology·Donovan H ParksGene W Tyson
Oct 27, 2017·PloS One·Brian BushnellEsther Singer
Nov 7, 2017·Nucleic Acids Research·Terje KlemetsenNils P Willassen
Jan 18, 2018·Scientific Data·Benjamin J TullyJohn F Heidelberg
Aug 28, 2018·Nature Biotechnology·Donovan H ParksPhilip Hugenholtz
Sep 5, 2018·Scientific Data·Steven J BillerSallie W Chisholm

Citations

Feb 21, 2020·FEMS Microbiology Reviews·S Joke LambrechtWolfgang R Hess
Feb 9, 2020·The ISME Journal·M C Muñoz-MarínJ M García-Fernández
Jan 15, 2021·Microbiology and Molecular Biology Reviews : MMBR·Ryan K FrittsJames B McKinlay

Methods Mentioned

BETA
flow cytometry

Related Concepts

Dimethylpropiothetin hydrochloride
Seawater
Sulfonium Compounds
Coculture Techniques
Alphaproteobacteria
Prochlorococcus
Heterotrophy
Betaine
Carbon
Genome

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.