Co-culture of two MDCK strains with distinct junctional protein expression: a model for intercellular junction rearrangement and cell sorting

Cell and Tissue Research
C B Collares-BuzatoN L Simmons

Abstract

Distinct epithelial MDCK cell strains displaying extremes in transepithelial electrical resistance (paracellular permeability) have been established in co-culture and the subsequent cellular behaviour and formation of junctional complexes investigated. After high-density seeding, MDCK strain I and II cells in co-culture are initially randomly distributed but subsequently sort themselves out in a time-dependent manner to form separate homotypic aggregates. The final pattern of cell arrangement of homotypic aggregates depends on the relative seeding proportion of each cell type. Immunostaining of established marker proteins for junctional complexes has revealed that MDCK I and II cells differ in the degree of expression of the zonula-adherens-associated protein, E-cadherin, their cytoskeletal architecture and the junctional distribution of a desmosomal protein, and by showing subtle differences in tight junction staining for the zona-occludens-associated proteins, ZO-1 and occludin. The distinct pattern of junctional protein expression is maintained when the two MDCK strains are co-cultured; however, morphologically atypical intercellular junctions between heterotypic cells at the boundary of homotypic cell aggregates have been o...Continue Reading

Citations

Jun 19, 2004·Computer Methods in Biomechanics and Biomedical Engineering·G Wayne Brodland, Colin J Wiebe
Oct 11, 2011·BMC Cell Biology·Joseph D DukesAndrew D Chalmers
Jan 10, 2009·Cell and Tissue Research·Thomaz A A Rocha-e-SilvaStephen Hyslop
Jun 21, 2012·Canadian Journal of Physiology and Pharmacology·Junia Carolina Santos-SilvaCarla Beatriz Collares-Buzato
Oct 28, 2015·The Journal of Cell Biology·Nivetha Kannan, Vivian W Tang
Jan 9, 2007·Toxicon : Official Journal of the International Society on Toxinology·Daniela C S DamicoCarla B Collares-Buzato
Jun 8, 2002·Toxicology and Applied Pharmacology·Carla B Collares-BuzatoMaria Alice da Cruz-Höfling
Oct 15, 2008·Physical Review Letters·M Shane HutsonDenis Viens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.