Co-culture with intestinal epithelial organoids allows efficient expansion and motility analysis of intraepithelial lymphocytes

Journal of Gastroenterology
Kengo NozakiTetsuya Nakamura

Abstract

Intraepithelial lymphocytes (IELs) in the intestine play important roles in the regulation of local immune responses. Although their functions have been studied in a variety of animal experiments, in vitro studies on spatiotemporal behaviors of IELs and their interaction with intestinal epithelial cells (IECs) have been hampered due to the lack of a suitable culture system. In this study, we aimed at developing a novel co-culture system of IELs with IECs to investigate dynamic interaction between these two populations of cells in vitro. We optimized experimental conditions under which murine IELs can be efficiently maintained with IECs cultured as three-dimensional organoids. We then tested the effect of IL-2, IL-7, and IL-15 on the maintenance of IELs in this co-culture system. By time-lapse imaging, we also examined the dynamic behaviors of IELs. IELs can be expanded with epithelial organoids in the presence of IL-2, IL-7, and IL-15. IELs were efficiently maintained within and outside of organoids showing a ~four-fold increase in both αβT and γδT IELs for a period of 2 weeks. Four-dimensional fluorescent imaging revealed an active, multi-directional movement of IELs along the basolateral surface of IECs, and also their inward...Continue Reading

References

Dec 1, 1990·European Journal of Immunology·J L Viney, T T MacDonald
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·M KohyamaH Ishikawa
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S MüllerC Mueller
Jul 21, 2001·Cell Death and Differentiation·T BrunnerC Frutschi
Oct 31, 2001·Nature Immunology·A HaydayJ Shires
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaping ChenRichard Boismenu
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anna BasAdrian C Hayday
Mar 30, 2011·Genesis : the Journal of Genetics and Development·Takaya AbeToshihiko Fujimori
Jun 18, 2011·Nature Reviews. Immunology·Hilde CheroutreDaniel Mucida
Apr 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen L EdelblumJerrold R Turner
Feb 26, 2014·Nature Reviews. Immunology·Lance W Peterson, David Artis

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Citations

Oct 22, 2016·Current Opinion in Cell Biology·Pawel J Schweiger, Kim B Jensen
Sep 23, 2016·Current Colorectal Cancer Reports·Madeleine Young, Karen R Reed
Sep 13, 2016·Diabetes, Obesity & Metabolism·A Grapin-Botton
Oct 8, 2016·Frontiers in Pharmacology·Fangkun LiuWei Zhao
Sep 16, 2016·Journal of Pediatric Gastroenterology and Nutrition·Thomas E Wallach, James R Bayrer
Mar 15, 2018·Current Opinion in Pediatrics·Genia Dubrovsky, James C Y Dunn
Dec 16, 2017·Current Pharmacology Reports·Madeleine D Hu, Karen L Edelblum
Dec 21, 2017·Frontiers in Microbiology·Nino Iakobachvili, Peter J Peters
Mar 16, 2017·Development·Jarno Drost, Hans Clevers
Jun 10, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madeleine D HuKaren L Edelblum
Dec 21, 2018·Omics : a Journal of Integrative Biology·Kevin DzoboM Iqbal Parker
Aug 17, 2018·Annual Review of Pharmacology and Toxicology·Toshio Takahashi
Aug 2, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Noriko WatanabeNaohiro Terada
Jun 20, 2019·American Journal of Physiology. Cell Physiology·Mohammad AlmeqdadiÖmer H Yilmaz
Jul 10, 2019·The EMBO Journal·Margit BleijsJarno Drost
Sep 17, 2018·Journal of Hematology & Oncology·Hanxiao XuKongming Wu
Nov 22, 2019·Briefings in Bioinformatics·Hongcheng YaoMulin Jun Li
May 1, 2019·The EMBO Journal·Theresa E SchnalzgerHenner F Farin
Feb 27, 2020·Nature Reviews. Gastroenterology & Hepatology·Harry Cheuk Hay LauJun Yu
Aug 9, 2017·Current Opinion in Pulmonary Medicine·Roderick H J HouwenMichael Wilschanski
Aug 28, 2020·Immunological Reviews·Matthew A FischerKaren L Edelblum
Jan 16, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H-D LiuG Lou
May 18, 2017·Journal of Immunology Research·Tanja ŠuligojBorut Božič
Dec 15, 2017·United European Gastroenterology Journal·Manuel NobenMarc Ferrante
Nov 14, 2017·Current Colorectal Cancer Reports·Apple G LongKathryn E Hamilton
Dec 26, 2017·Current Colorectal Cancer Reports·Sarah P ShortChristopher S Williams
Feb 28, 2019·Stem Cells International·Judith Kraiczy, Matthias Zilbauer
Dec 20, 2019·Nature Reviews. Immunology·Yotam E Bar-EphraimHans Clevers
Dec 20, 2019·Nature Protocols·Chiara M CattaneoEmile E Voest
May 18, 2020·Mucosal Immunology·Maud VandereykenMahima Swamy
Mar 30, 2020·Diabetologia·Anastasia TsakmakiGavin A Bewick
Jul 9, 2020·Nature Reviews. Molecular Cell Biology·Jihoon KimJuergen A Knoblich
Sep 22, 2020·Cellular and Molecular Bioengineering·Benjamin C Hacker, Marjan Rafat
Dec 7, 2019·Stem Cells and Development·Lauren O'Connell, Des C Winter
Mar 21, 2019·Frontiers in Cellular and Infection Microbiology·Estefania Delgado BetancourtFrank Seeber
Aug 19, 2020·Nature Materials·Masayuki Fujii, Toshiro Sato
Apr 26, 2018·Nature Reviews. Cancer·Jarno Drost, Hans Clevers
Nov 16, 2018·Gastroenterology Report·Huayu YangYilei Mao
Nov 12, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·John D O'NeillGordana Vunjak-Novakovic

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Methods Mentioned

BETA
IECs
FACS
IELs
flow cytometry
confocal microscopy

Software Mentioned

Imaris
SoftWorx
Adobe Photoshop

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