Co-cultured hBMSCs and HUVECs on human bio-derived bone scaffolds provide support for the long-term ex vivo culture of HSC/HPCs

Journal of Biomedical Materials Research. Part a
Xiao-Bing HuangLingling Wei

Abstract

In order to closely mimic a multi-cell state in hematopoietic stem/progenitor cells (HSC/HPCs) vascular niche, we co-cultured human bone marrow mesenchymal stem cells (hBMSCs) and human umbilical vein endothelial cells (HUVECs) without any cytokines as feeder cells and applied bio-derived bone from human femoral metaphyseal portion as scaffold to develop a new HSC/HPCs three-dimensional culture system (named 3D-Mix cultures). Scanning electron and fluorescent microscopy showed excellent biocompatibility of bio-derived bone to hBMSCs and HUVECs in vitro. Flow cytometry analysis and quantitative real-time polymerase chain reaction (qPCR) assay of p21 expression demonstrated that 3D-Mix could promote self-renewal and ex vivo expansion of HSCs/HPCs significantly higher than 3D-hMSC and 3D-HUVEC. Long-term culture initiating cell (LTC-IC) confirmed that 3D-Mix had the most powerful activity of maintaining multipotent differentiation of primitive cell subpopulation in HSCs. The nonobese diabetic/severe combined immunodeficiency (NOD/SCID) repopulating cell (SRC) assay demonstrated that 3D-Mix promoted the expansion of long-term primitive transplantable HSCs. qPCR of alkaline phosphatase (ALP) and osteocalcin (OC) demonstrated that HU...Continue Reading

References

Jun 18, 1999·Annals of the New York Academy of Sciences·R MöhleL Kanz
Sep 21, 2000·Journal of Cellular Biochemistry·F VillarsJ Amédée
Mar 7, 2002·American Journal of Physiology. Cell Physiology·F VillarsJ Amédée
Oct 24, 2003·Nature·L M CalviD T Scadden
Nov 17, 2004·Genes & Development·Juan GalceranRudolf Grosschedl
Jan 25, 2005·Nature Immunology·Andrew W DuncanTannishtha Reya
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Darnell KaiglerDavid J Mooney
Dec 19, 2006·Immunity·Mark J Kiel, Sean J Morrison
Apr 5, 2007·Nature Protocols·Bruno BaudinMichel Vaubourdolle
Sep 19, 2007·Journal of Gastroenterology·Tetsuya NakamuraMamoru Watanabe
Aug 12, 2009·International Journal of Hematology·Li HouYuchun Wang
Nov 19, 2009·Biomedical Engineering Online·Ying XueKamal Mustafa
Jun 12, 2010·Stem Cells and Development·Fatima A SalehPaul G Genever
Aug 17, 2010·The International Journal of Developmental Biology·Anna BigasLluís Espinosa
Feb 9, 2012·Journal of Materials Science. Materials in Medicine·Huixu XieLongjiang Li
Mar 29, 2012·Inflammation & Allergy Drug Targets·Tatsuki Sugiyama, Takashi Nagasawa
Oct 23, 2012·Nature Medicine·Ingrid G WinklerJean-Pierre Lévesque
Feb 1, 2013·Advances in Hematology·Felix Nwajei, Marina Konopleva
Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ted J HofmannEdwin M Horwitz
Apr 11, 2014·BioMed Research International·Muti ur Rehman KhanMeifu Feng

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Citations

Mar 5, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Ji Eun KimJung-Woog Shin
Feb 6, 2018·Advanced Pharmaceutical Bulletin·Hamze TimariSara Aqmasheh
Apr 23, 2016·Journal of Biomedical Materials Research. Part a·Aylin KomezVasif Hasirci
Jan 7, 2020·Nature Reviews. Materials·Sungwoong KimAnkur Singh
Mar 9, 2021·Tissue Engineering. Part B, Reviews·Cláudia S OliveiraJoão F Mano
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhi TangHui Xiong

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