Co-Cultures of Mycorrhizal Fungi Do Not Increase Germination and Seedling Development in the Epiphytic Orchid Dendrobium nobile

Frontiers in Plant Science
Shi-Cheng ShaoHans Jacquemyn

Abstract

Orchids are highly dependent on mycorrhizal fungi for seed germination and subsequent growth to a seedling as they provide essential carbon, water, and mineral nutrients to developing seeds. Although there is mounting evidence that orchid seeds are often colonized by multiple fungi simultaneously, most in vitro germination experiments focus on mycorrhizal monocultures and little is known about how mycorrhizal assemblages affect seed germination and growth of seedlings. In this study, we compared the effects of mycorrhizal monocultures and co-cultures on seed germination and seedling growth of the epiphytic orchid Dendrobium nobile. In situ baiting was used to isolate mycorrhizal fungi from protocorms for germination experiments. Germination experiments were conducted under two light regimes for 90 days. In total, five fungal strains were isolated from protocorms of D. nobile, indicating that the species was not highly specific to its fungal partners. Four strains (JC-01, JC-02, JC-04, and JC-05) belonged to the Serendipitaceae and one (JC-03) to the Tulasnellaceae. In vitro germination experiments showed that germination percentages were higher under light-dark conditions than under complete dark conditions, supporting previous...Continue Reading

References

Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Jul 17, 2008·Molecular Ecology·Martin I Bidartondo, David J Read
Sep 8, 2009·Trends in Plant Science·Nigel D Swarts, Kingsley W Dixon
Aug 15, 2015·Annals of Botany·Hanne N RasmussenTamara Těšitelová
Sep 16, 2015·Conservation Biology : the Journal of the Society for Conservation Biology·Qiang LiuJiangYun Gao
Feb 3, 2016·The New Phytologist·Gerhard GebauerAndreas C Gebauer
May 20, 2016·The New Phytologist·Michael WeißMarc-André Selosse
Jan 19, 2017·Molecular Ecology·Michael WaudHans Jacquemyn
Mar 17, 2017·Science China. Life Sciences·Xiaoke XingShunxing Guo
Mar 24, 2017·Annals of Botany·Julienne M-I SchieboldGerhard Gebauer
Jun 24, 2017·Frontiers in Plant Science·Shi-Cheng ShaoJiang-Yun Gao
Feb 18, 2018·Ecology·Rebecca T BatstoneMegan E Frederickson
May 24, 2018·The New Phytologist·Melissa K McCormickArmando Canchani-Viruet

❮ Previous
Next ❯

Datasets Mentioned

BETA
MH500253
MH500251-MH500255

Methods Mentioned

BETA
PCR

Software Mentioned

FigTree
PAUP
SPSS
MrBayes
BioEdit
Clustal X

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.