Co-cultures of Pseudomonas aeruginosa and Roseobacter denitrificans reveal shifts in gene expression levels compared to solo cultures.

TheScientificWorldJournal
Crystal A ConwayJose V Lopez

Abstract

Consistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable. In a proof-of-principle study to induce SM gene expression and production, we describe mixed "co-culturing" conditions and monitoring of messages via quantitative real-time PCR (qPCR). Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interactions on gene expression in vitro, Two P. aeruginosa genes (PhzH coding for portions of the phenazine antibiotic pathway leading to pyocyanin (PCN) and the RhdA gene for thiosulfate: cyanide sulfurtransferase (Rhodanese)) and two R. denitrificans genes (BetaLact for metallo-beta-lactamase and the DMSP gene for dimethylpropiothetin dethiomethylase) were assessed for differential expression. Results showed that R. denitrificans DMSP and BetaLact gene expression became elevated in a mixed culture. In contrast, P. aeruginosa co-cultures with R. denitrificans or a third species did not increase target gene expression above control levels. This paper provides insight for better control of target SM gene expression in vitro and bypass complex ge...Continue Reading

References

Dec 23, 1999·Current Opinion in Microbiology·B L Bassler
Mar 14, 2001·Natural Product Reports·D J Faulkner
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 21, 2002·Natural Product Reports·D John Faulkner
Oct 6, 2005·Applied and Environmental Microbiology·Alison BuchanMary Ann Moran
Feb 28, 2006·Nature Reviews. Microbiology·Laurent Keller, Michael G Surette
May 25, 2006·Annual Review of Phytopathology·Dmitri V MavrodiLinda S Thomashow
Nov 14, 2006·Applied and Environmental Microbiology·Rita CipollonePaolo Visca
May 15, 2007·Microbial Ecology·Albrecht Muscholl-SilberhornJohannes F Imhoff
Oct 31, 2008·The ISME Journal·Nicole S Webster, Linda L Blackall
Jan 14, 2009·Applied and Environmental Microbiology·Yongyu Zhang, Nianzhi Jiao
May 16, 2009·Nature·Edward F DeLong
Aug 21, 2009·Environmental Microbiology·Krystal L RypienFarooq Azam
Oct 2, 2009·HFSP Journal·Vittorio Venturi, Sujatha Subramoni
Dec 1, 2009·Nature Reviews. Microbiology·Michael E HibbingS Brook Peterson
May 22, 2010·Science·Daniel G GibsonJ Craig Venter
Jul 27, 2010·Journal of Bacteriology·Floyd E DewhirstWilliam G Wade

❮ Previous
Next ❯

Datasets Mentioned

BETA
AE004091
CP000362

Methods Mentioned

BETA
PCR

Software Mentioned

GeneX
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.