Co-enzyme Q10 and acetyl salicylic acid enhance Hsp70 expression in primary chicken myocardial cells to protect the cells during heat stress

Molecular and Cellular Biochemistry
J XuE Bao

Abstract

We investigated the effects of co-enzyme Q10 (Q10) and acetyl salicylic acid (ASA) on expression of Hsp70 in the protection of primary chicken myocardial cells during heat stress. Western blot analysis showed that Q10 and ASA accelerated the induction of Hsp70 when chicken myocardial cells were exposed to hyperthermia. In the absence of heat stress, however, neither Q10 nor ASA are able to upregulate Hsp70 expression. Analysis of enzymes that respond to cellular damage and pathological examination revealed that ectopic expression of ASA and Q10 alleviate cellular damage during heat stress. Quantification of heat shock factors (HSF) indicated that treatment of ASA increased the expression of HSF-1 and HSF-3 during heat stress. Treatment with Q10 resulted in the elevation of HSF-1 expression. Expression of HSF-2 and HSF-4 was not affected by ASA or Q10. Subcellular distribution analysis of HSF-1 and HSF-3 showed that in response to heat stress ASA promoted nuclear translocation of HSF-1 and HSF-3, while Q10 promoted only HSF-1 nuclear translocation. Chromatin immunoprecipitation (ChIP) analysis indicated that HSF-1 occupies the Hsp70 promoter in chicken primary myocardial cells during heat stress and under normal conditions, whil...Continue Reading

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Citations

Nov 15, 2018·Oxidative Medicine and Cellular Longevity·Shu TangEndong Bao
Jun 18, 2020·International Journal of Molecular Sciences·Dongyang WangJiajie Sun
Aug 21, 2021·Oxidative Medicine and Cellular Longevity·Bin YinGuiming Li

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Methods Mentioned

BETA
nuclear translocation
electrophoresis
PCR
MDA
immunoprecipitation assay
immunoprecipitation
ChIP

Software Mentioned

Quantity One
SPSS

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