Co-existence of BRAFV600E and TERT promoter mutations in papillary thyroid carcinoma is associated with tumor aggressiveness, but not with lymph node metastasis

Cancer Management and Research
Haoyu RenXinliang Su

Abstract

Mutations of BRAFV600E and TERT promoters are associated with thyroid cancer development. This study further investigated association of these mutations with clinicopathological characteristics from patients with papillary thyroid carcinoma (PTC). Tumor tissues from 342 PTC patients were obtained for DNA extraction and polymerase chain reaction amplification to detect the BRAFV600E mutation using amplification-refractory mutation system-polymerase chain reaction. TERT promoter mutations were assessed using Sanger DNA sequencing. The association of these gene mutations with clinicopathological characteristics was then statistically analyzed. Two hundred and seventy of 342 (78.9%) PTC patients harbored the BRAFV600E mutation, which was associated with older age male patients. Moreover, TERT promoter mutations occurred in 12 of 342 (3.5 %) PTC patients, all of whom also had the BRAF mutation. One hundred thirty-three patients with papillary thyroid microcarcinoma (PTMC) had no TERT mutations. Statistically, the coexistence of BRAF and TERT promoter mutations were significantly associated with older age, larger tumor size, extrathyroidal extension, and advanced tumor stage, but not with central lymph node metastasis, lateral lymph ...Continue Reading

Citations

Apr 24, 2019·Diagnostic Cytopathology·John Woody SistrunkEdward Grant
Sep 25, 2019·Omics : a Journal of Integrative Biology·Sevim Dalva-AydemirMustafa Cengiz Yakıcıer
Jun 7, 2019·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Mu LiAhmad Daniyal Siddiqui
Jul 9, 2021·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Mengying TongYing Che

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Methods Mentioned

BETA
dissections
PCR
electrophoresis
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Software Mentioned

SPSS

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