Co-exposure to the food additives SiO2 (E551) or TiO2 (E171) and the pesticide boscalid increases cytotoxicity and bioavailability of the pesticide in a tri-culture small intestinal epithelium model: Potential health implications

Environmental Science. Nano
Xiaoqiong CaoPhilip Demokritou

Abstract

Many toxicity investigations have evaluated the potential health risks of ingested engineered nanomaterials (iENMs); however, few have addressed the potential combined effects of iENMs and other toxic compounds (e.g. pesticides) in food. To address this knowledge gap, we investigated the effects of two widely used, partly nanoscale, engineered particulate food additives, TiO2 (E171) and SiO2 (E551), on the cytotoxicity and cellular uptake and translocation of the pesticide boscalid. Fasting food model (phosphate buffer) containing iENM (1% w/w), boscalid (10 or 150 ppm), or both, was processed using a simulated in vitro oral-gastric-small intestinal digestion system. The resulting small intestinal digesta was applied to an in vitro tri-culture small intestinal epithelium model, and effects on cell layer integrity, viability, cytotoxicity and production of reactive oxygen species (ROS) were assessed. Boscalid uptake and translocation was also quantified by LC/MS. Cytotoxicity and ROS production in cells exposed to combined iENM and boscalid were greater than in cells exposed to either iENM or boscalid alone. More importantly, translocation of boscalid across the tri-culture cellular layer was increased by 20% and 30% in the pres...Continue Reading

References

Sep 17, 2008·Experimental & Applied Acarology·Noubar J BostanianGaétan Racette
Feb 10, 2009·Journal of Nanoscience and Nanotechnology·Soo Jeong SoChong Soo Han
Aug 21, 2010·Journal of Hazardous Materials·Yaling CuiFashui Hong
Sep 28, 2010·Nanotoxicology·Susan DekkersAgnes G Oomen
Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Yaling CuiFashui Hong
Jan 18, 2011·The Science of the Total Environment·Demin WangJianmin Wang
May 14, 2011·Environmental Pollution·Demin WangJianmin Wang
May 20, 2011·Journal of Agricultural and Food Chemistry·Alberto AngioniPierluigi Caboni
Sep 13, 2011·Journal of Hazardous Materials·Suxing GuiFashui Hong
Jan 21, 2012·Environmental Science & Technology·Alex WeirNatalie von Goetz
Feb 14, 2012·Nature Nanotechnology·Gretchen J MahlerMichael L Shuler
Aug 21, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nathalie Zucchini-PascalRoger Rahmani
Jul 17, 2013·Journal of Hazardous Materials·Mingchun GuoShanshan Zhu
Feb 1, 2014·Journal of Agricultural and Food Chemistry·Hongda ChenMatt Hotze
Apr 8, 2016·Critical Reviews in Food Science and Nutrition·Aditi JainChidambaram Ramalingam
Dec 20, 2016·Current Opinion in Biotechnology·Mary EleftheriadouPhilip Demokritou
Jan 20, 2017·Nature Protocols·Glen M DeLoidPhilip Demokritou
May 31, 2018·Analytical and Bioanalytical Chemistry·Ju Yong LeeWenwan Zhong
Jul 4, 2018·Small·Magdiel Inggrid SetyawatiDavid Tai Leong
Jul 5, 2018·Particle and Fibre Toxicology·Ikjot Singh SohalDhimiter Bello

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Citations

Jun 5, 2020·Small·Magdiel Inggrid SetyawatiKee Woei Ng
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sung Bum ParkByumseok Koh
Dec 10, 2020·Journal of Hazardous Materials·Caixia GuoYanbo Li

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Methods Mentioned

BETA
transmission electron microscopy
X-ray
dynamic light scattering
protein assay
infrared spectroscopy

Software Mentioned

NanoAnalyze
Prism
GraphPad

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