Co-expression of elastin and collagen leads to highly compliant engineered blood vessels

Journal of Biomedical Materials Research. Part a
J GaoYadong Wang

Abstract

Elastin synthesis and physiologic compliance are significant challenges in blood vessel tissue engineering. Here, we report that a biocompatible elastomeric scaffold can support the co-expression of elastin and collagen, which likely yielded the physiologic compliance in the constructs. A biodegradable elastomer, poly(glycerol sebacate), was fabricated into highly porous tubular scaffolds. Primary baboon arterial smooth muscle cells (SMCs) were seeded in the lumen of the scaffolds followed by a 1-week culture under gentle perfusion. Circulating endothelial progenitor cells (EPCs) isolated from baboon peripheral blood was seeded directly on the smooth muscle layer in the lumen on day 8. The constructs were perfused using a pulsatile flow system for another 2 weeks before characterization. In another set of experiments, the SMCs were cultured for 7 weeks and were co-cultured for 1 week with the EPCs. Constructs obtained using either set of culture conditions contained elastin and collagen: Masson's trichrome stain showed a circumferential collagen band in the constructs, and elastin was evident from its characteristic autofluorescence, Verhoff's stain, and amino acid analysis of insoluble remnants after hot alkali digestion. All ...Continue Reading

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Citations

Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kee-Won LeeYadong Wang
Jan 10, 2012·Tissue Engineering. Part B, Reviews·Chris A BashurAnand Ramamurthi
Dec 10, 2013·Tissue Engineering. Part a·Lavanya VenkataramanAnand Ramamurthi
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