Co-expression of P173S Mutant Rice EPSPS and igrA Genes Results in Higher Glyphosate Tolerance in Transgenic Rice

Frontiers in Plant Science
Dhirendra FartyalMalireddy K Reddy

Abstract

Weeds and their devastating effects have been a great threat since the start of agriculture. They compete with crop plants in the field and negatively influence the crop yield quality and quantity along with survival of the plants. Glyphosate is an important broad-spectrum systemic herbicide which has been widely used to combat various weed problems since last two decades. It is very effective even at low concentrations, and possesses low environmental toxicity and soil residual activity. However, the residual concentration of glyphosate inside the plant has been of major concern as it severely affects the important metabolic pathways, and results in poor plant growth and grain yield. In this study, we compared the glyphosate tolerance efficiency of two different transgenic groups over expressing proline/173/serine (P173S) rice EPSPS glyphosate tolerant mutant gene (OsmEPSPS) alone and in combination with the glyphosate detoxifying encoding igrA gene, recently characterized from Pseudomonas. The molecular analysis of all transgenic plant lines showed a stable integration of transgenes and their active expression in foliar tissues. The physiological analysis of glyphosate treated transgenic lines at seed germination and vegetati...Continue Reading

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Citations

Jan 23, 2019·Environmental Microbiology·Dennis WickeFabian M Commichau
Oct 28, 2019·Transgenic Research·Xu-Jing WangZhi-Xing Wang
Apr 21, 2021·Environmental Microbiology·Robert HertelFabian M Commichau

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Methods Mentioned

BETA
transgenics
transgenic
PCR
electrophoresis

Software Mentioned

MEGA
BLAST

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