Co-expression of squalene epoxidases with triterpene cyclases boosts production of triterpenoids in plants and yeast

Metabolic Engineering
Lemeng DongSøren Bak

Abstract

Triterpene cyclases catalyze the first committed step in triterpene biosynthesis, by forming mono- to pentacyclic backbone structures from oxygenated C30 isoprenoid precursors. Squalene epoxidase precedes this cyclization by providing the oxygenated and activated substrate for triterpene biosynthesis. Three squalene epoxidases from Cucurbita pepo (CpSEs) were isolated and shown to have evolved under purifying selection with signs of sites under positive selection in their N- and C-termini. They all localize to the Endoplasmic Reticulum (ER) and produce 2,3-oxidosqualene and 2,3:22,23-dioxidosqualene when expressed in a yeast erg1 (squalene epoxidase) erg7 (lanosterol synthase) double mutant. Co-expression of the CpSEs with four different triterpene cyclases, either transiently in Nicotiana benthamiana or constitutively in yeast, showed that CpSEs boost triterpene production. CpSE2 was the best performing in this regard, which could reflect either increased substrate production or superior channeling of the substrate to the triterpene cyclases. Fluorescence Lifetime Imaging Microscopy (FLIM) analysis with C. pepo cucurbitadienol synthase (CpCPQ) revealed a specific interaction with CpSE2 but not with the other CpSEs. When CpSE2 ...Continue Reading

References

Jul 10, 1974·Biochemical and Biophysical Research Communications·F Karst, F Lacroute
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Varshavsky
Aug 22, 2001·Protein Science : a Publication of the Protein Society·O Dym, D Eisenberg
Jun 21, 2002·Methods in Enzymology·R Daniel Gietz, Robin A Woods
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Hideyuki SuzukiRichard A Dixon
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Mar 29, 2007·Nucleic Acids Research·Fernando Geu-FloresBarbara A Halkier
Apr 12, 2007·The Journal of Biological Chemistry·Jeanne M RasberyBonnie Bartel
Nov 17, 2007·Journal of Natural Products·Teresa MencheriniRita Aquino
Jan 22, 2008·The Plant Cell·Panagiota MylonaAnne Osbourn
Jan 24, 2008·Antimicrobial Agents and Chemotherapy·Christoph RuckenstuhlFriederike Turnowsky
May 29, 2010·Science·Susanna SeppäläGunnar von Heijne
Sep 28, 2010·The New Phytologist·Costas DelisKalliope K Papadopoulou
Aug 2, 2011·Progress in Lipid Research·John S Burg, Peter J Espenshade
Jun 26, 2012·Planta·Shuiqin WuJoe Chappell
Mar 6, 2013·Nucleic Acids Research·James E DiCarloGeorge M Church
Oct 10, 2013·Molecular Biology and Evolution·Bo Xu, Ziheng Yang
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tessa MosesAlain Goossens
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ariane C KemenAnne Osbourn
Mar 21, 2016·Biotechnology Advances·Pan LiaoMee-Len Chye
Jul 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Melissa SalmonAnne Osbourn
Jul 28, 2016·Plant Science : an International Journal of Experimental Plant Biology·Jennifer Fiallos-JuradoAntonio Leon-Reyes
Feb 22, 2017·Metabolic Engineering·Philipp ArendtAlain Goossens
Dec 6, 2017·Plant Physiology·Aldo AlmeidaSøren Bak

❮ Previous
Next ❯

Citations

Jul 26, 2019·The Plant Journal : for Cell and Molecular Biology·Eva NiephausChristian Schulze Gronover
Apr 21, 2020·Biotechnology and Bioengineering·Pisanee SrisawatToshiya Muranaka
Nov 5, 2019·Scientific Reports·Zahra AminfarMohammad Hossein Mirjalili
Nov 28, 2018·Nature Microbiology·Jacob PollierMichele Fabris
Jun 5, 2020·BMC Plant Biology·Pan ZhangXiaochun Zhao
Jul 16, 2021·Molecular Biology and Evolution·Lemeng DongSøren Bak
Aug 28, 2021·ACS Synthetic Biology·Weibo QiaoYansheng Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.