PMID: 9180158May 29, 1997Paper

Co-expression of urokinase-type plasminogen activator and its receptor in human gastric-cancer cell lines correlates with their invasiveness and tumorigenicity

International Journal of Cancer. Journal International Du Cancer
I K ParkY H Kook

Abstract

The expression of urokinase-type plasminogen activator (u-PA), its receptor (u-PAR) and metalloproteases activity were analyzed in 4 human gastric-cancer cell lines (AGS, Hs746T, SNU-1, and SNU-5), in an attempt to relate these activities to their invasive potential and tumorigenicity on the modified chorioallantoic membranes (CAM) of chick embryos. Only 1 of the 4 cell lines tested, Hs746T, expressed both u-PA and u-PAR as well as MMP-2, but not MMP-9. This cell line was both tumorigenic and highly invasive (51.3 +/- 13.1%) on a modified CAM. Its invasive capacity was comparable with that of a highly malignant human epidermoid-carcinoma cell line (HEp3), which usually showed 40 to 50% invasiveness. The 3 other cell lines all produced MMP-2 and MMP-9, but only AGS showed moderate invasiveness (24.2 +/- 8.8%). While antibodies to u-PA were significantly effective in reducing CAM invasiveness of Hs746T cells by approximately 40%, the invasiveness of the t-PA-expressing AGS cell line was not affected by anti-t-PA antibodies. These results suggest that when one of the components of the u-PA/u-PAR system (the enzyme and/or the receptor) is not produced and u-PA/u-PAR-dependent cell-surface proteolytic activity is thereby diminished,...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E BacharachE Keshet
Jul 1, 1991·Seminars in Thrombosis and Hemostasis·H C KwaanR Ernst
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M P StoppelliR K Assoian
Feb 1, 1988·Gastroenterologia Japonica·H KarasawaT Miwa
Jun 15, 1995·Biochemical and Biophysical Research Communications·S JimiH Ishikawa
Aug 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M M HeissF W Schildberg
Jan 1, 1995·Acta Anatomica·M Flug, P Köpf-Maier
Apr 28, 1995·The Journal of Biological Chemistry·S J MandriotaM S Pepper
Jul 1, 1994·Clinical & Experimental Metastasis·T AbeM Kuwano
Jan 1, 1994·Trends in Pharmacological Sciences·F Fazioli, F Blasi
Jan 1, 1994·Cancer·G K SchwartzA P Albino

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Citations

Feb 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Ja-Lok Ku, Jae-Gahb Park
Feb 25, 2011·Annals of Surgical Oncology·Hiroshi SakaiMasao Tanaka
Apr 21, 2010·Expert Opinion on Investigational Drugs·Ralf HildenbrandPhilipp Stroebel
Apr 25, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Tian Tian GuoBoon Huat Bay
Oct 10, 2013·International Journal of Cancer. Journal International Du Cancer·Shenghong ZhangJifan Hu
Oct 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yih-Shou HsiehPei-Ni Chen

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