Co-expression of VGLUT1 and VGAT sustains glutamate and GABA co-release and is regulated by activity in cortical neurons

Journal of Cell Science
G FattoriniF Conti

Abstract

In adult neocortex, VGLUT1 (also known as SLC17A7), the main glutamate vesicular transporter, and VGAT (also known as SLC32A1), the γ-aminobutyric acid (GABA) vesicular transporter, are co-expressed in a subset of axon terminals forming both symmetric and asymmetric synapses, where they are sorted into the same vesicles. However, the functional consequence of this colocalization in cortical neurons has not been clarified. Here, we tested the hypothesis that cortical axon terminals co-expressing VGLUT1 and VGAT can evoke simultaneously monosynaptic glutamate and GABA responses, and investigated whether the amount of terminals co-expressing VGLUT1 and VGAT is affected by perturbations of excitation-inhibition balance. In rat primary cortical neurons, we found that a proportion of synaptic and autaptic responses were indeed sensitive to consecutive application of selective glutamate and GABAA receptor blockers. These 'mixed' synapses exhibited paired-pulse depression. Notably, reducing the activity of the neuronal network by treatment with glutamate receptor antagonists decreased the amount of 'mixed' synapses, whereas reducing spontaneous inhibition by treatment with bicuculline increased them. These synapses might contribute to ...Continue Reading

References

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Citations

Feb 26, 2016·Frontiers in Synaptic Neuroscience·Agnieszka Münster-WandowskiGudrun Ahnert-Hilger
Jan 15, 2016·Journal of Chemical Neuroanatomy·David J BarkerMarisela Morales
Oct 26, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Mikaela TherrienThomas B Shea
May 28, 2019·Molecular Neurobiology·Julianna KardosLászló Héja
Aug 30, 2017·Frontiers in Cellular Neuroscience·Giorgia FattoriniFiorenzo Conti
Feb 16, 2020·The Journal of Comparative Neurology·Hannah M McKinneyR Steven Stowers
Sep 9, 2015·Stem Cell Research & Therapy·Raffaella MoriniMichela Matteoli
Apr 19, 2019·ELife·Matthew J KourakisWilliam C Smith

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