Co-immobilization of ACH11 antithrombotic peptide and CAG cell-adhesive peptide onto vascular grafts for improved hemocompatibility and endothelialization

Acta Biomaterialia
Jing ZhaoYakai Feng

Abstract

Surface modification by conjugating biomolecules has been widely proved to enhance biocompatibility of small-caliber artificial vascular grafts. In this study, we aimed at developing a multifunctional vascular graft that provides not only good hemocompatibility but also in situ rapid endothelialization. Herein, a vascular graft (inner diameter ∼2 mm) was fabricated by electrospinning with poly(lactic acid-co-caprolactone) and gelatin, and then biofunctionalized with antithrombotic peptide with sequence LTFPRIVFVLG (ACH11) and cell adhesion peptide with sequence CAG through adhesive poly(dopamine) coating. We developed this graft with the synergistic properties of low thrombogenicity and rapid endothelialization. The successful grafting of both CAG and ACH11 peptides was confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The surface micromorphology of the modified surfaces was observed by field emission scanning electron microscopy. Our results demonstrated that the multifunctional surface suppressed the denaturation of absorbed fibrinogen, hindered coagulation factor Xa activation, and inhibited platelet adhesion and aggregation. Importantly, this modified surface could selectively enhanc...Continue Reading

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Citations

Dec 6, 2019·Frontiers in Bioengineering and Biotechnology·Alicia Fernández-ColinoPetra Mela
Jan 12, 2021·Advanced Drug Delivery Reviews·Jun ChenHo-Wook Jun
Oct 3, 2020·Bioactive Materials·Ting SuJianliang Shen
Dec 17, 2020·Acta Biomaterialia·Raffaella BucciFrancesca Clerici
Apr 1, 2021·ACS Applied Materials & Interfaces·Jiaying ZhouYakai Feng
Apr 20, 2021·Advanced Healthcare Materials·Chao Tao, Dong-An Wang
Mar 3, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Tianyu ZhuJian Shen
Oct 16, 2021·Journal of Agricultural and Food Chemistry·Shuhui ZhangAimin Ma

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