PMID: 7541688Apr 1, 1995Paper

Co-induction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids

British Journal of Pharmacology
T A SwierkoszJ R Vane

Abstract

1. Lipopolysaccharide (LPS) co-induces nitric oxide synthase (iNOS) and cyclo-oxygenase (COX-2) in J774.2 macrophages. Here we have used LPS-activated J774.2 macrophages to investigate the effects of exogenous or endogenous nitric oxide (NO) on COX-2 in both intact and broken cell preparations. NOS activity was assessed by measuring the accumulation of nitrite using the Griess reaction. COX-2 activity was assessed by measuring the formation of 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) by radioimmunoassay. Western blot analysis was used to determine the expression of COX-2 protein. We have also investigated whether endogenous NO regulates the activity and/or expression of COX in vivo by measuring NOS and COX activity in the lung and kidney, as well as release of prostanoids from the perfused lung of normal and LPS-treated rats. 2. Incubation of cultured murine macrophages (J774.2 cells) with LPS (1 microgram ml-1) for 24 h caused a time-dependent accumulation of nitrite and 6-keto-PGF1 alpha in the cell culture medium which was first significant after 6 h. The formation of both 6-keto-PGF1 alpha and nitrite elicited by LPS was inhibited by cycloheximide (1 microM) or dexamethasone (1 microM). Western blot analysis showed...Continue Reading

References

Feb 1, 1992·British Journal of Pharmacology·J A MitchellJ R Vane
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Jan 1, 1992·Cardiovascular Research·C E WrightS Moncada
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J StuehrC F Nathan
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S PollockF Murad
Jun 1, 1991·British Journal of Pharmacology·J A MitchellJ R Vane
Nov 1, 1990·British Journal of Pharmacology·H A Bull, P M Dowd
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Jul 17, 1990·European Journal of Pharmacology·J A MitchellJ R Vane
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G de NucciJ R Vane
Jun 22, 1988·European Journal of Pharmacology·M G DoniS Moncada
Jan 1, 1988·European Heart Journal·K SchrörE König
Jan 1, 1985·Circulation·R De CaterinaB B Weksler
Jan 1, 1985·Journal of Free Radicals in Biology & Medicine·W E Lands
Oct 1, 1983·Journal of the American College of Cardiology·J MehtaL Brigmon
Mar 1, 1981·The Journal of Clinical Investigation·R I LevinK Tack-Goldman
May 1, 1994·The Journal of Clinical Investigation·D SalveminiP Needleman
Jan 1, 1994·Advances in Pharmacology·C Thiemermann
Sep 1, 1994·European Journal of Pharmacology·L Sautebin, M Di Rosa
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C ThiemermannJ R Vane
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SalveminiP Needleman
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V RettoriS M McCann
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M FranchiM Gimeno
Sep 1, 1994·Archives of Biochemistry and Biophysics·A L TsaiR J Kulmacz
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A MitchellJ R Vane
Jul 15, 1993·Biochemical and Biophysical Research Communications·T InoueT Ogihara

❮ Previous
Next ❯

Citations

Jul 15, 1998·Arthritis and Rheumatism·R M ClancyS B Abramson
Jan 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J R Vane, R M Botting
Jun 1, 1996·Intensive Care Medicine·N P CurzenJ A Mitchell
Mar 1, 1996·Immunology Today·K RackeI Wessler
May 11, 2005·Neuroscience Letters·C MarchiniE Fioretti
Mar 10, 2001·Biochemical Pharmacology·V M Dirsch, A M Vollmar
Nov 8, 2002·European Journal of Pharmacology·Hans GühringKay Brune
May 12, 1997·European Journal of Pharmacology·G BrunnK Racké
Feb 24, 1998·European Journal of Pharmacology·R A Lefebvre
Jul 22, 1998·European Journal of Pharmacology·R ZamoraA G Herman
Oct 2, 2001·Prostaglandins & Other Lipid Mediators·G Basini, C Tamanini
Feb 13, 1999·Progress in Neurobiology·M J Millan
May 20, 1998·Nutrition·B Alexander
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Benedetta CinqueM Grazia Cifone
May 29, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Bahar TunçtanNurettin Abacioglu
Dec 14, 2006·Prostaglandins & Other Lipid Mediators·Rita K UpmacisDavid P Hajjar
Jan 26, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·C BernardeauM Breban
Feb 1, 1997·British Journal of Pharmacology·B ZingarelliC Szabó
May 1, 1997·British Journal of Pharmacology·D Bishop-BaileyJ A Mitchell
Jun 16, 1999·British Journal of Pharmacology·G RupprechtM Kaszkin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.