Co-infection with Mycobacterium tuberculosis impairs HIV-Specific CD8+ and CD4+ T cell functionality

PloS One
Shivan ChettyVictoria O Kasprowicz

Abstract

The ability of antigen-specific T cells to simultaneously produce multiple cytokines is thought to correlate with the functional capacity and efficacy of T cells. These 'polyfunctional' T cells have been associated with control of HIV. We aimed to assess the impact of co-infection with Mycobacterium tuberculosis (MTB) on HIV-specific CD8+ and CD4+ T cell function. We assessed T cell functionality in 34 South African adults by investigating the IFN-y, IL-2, TNF-α, IL-21 and IL-17 cytokine secretion capacity, using polychromatic flow cytometry, following HIV Gag-specific stimulation of peripheral blood mononuclear cells. We show that MTB is associated with lower HIV-specific T cell function in co-infected as compared to HIV mono-infected individuals. This decline in function was greatest in co-infection with active Tuberculosis (TB) compared to co-infection with latent MTB (LTBI), suggesting that mycobacterial load may contribute to this loss of function. The described impact of MTB on HIV-specific T cell function may be a mechanism for increased HIV disease progression in co-infected subjects as functionally impaired T cells may be less able to control HIV.

References

Feb 21, 2002·The Journal of Experimental Medicine·Yoshihiko HoshinoMichael Weiden
Nov 13, 2008·The Journal of Infectious Diseases·Christof GeldmacherMichael Hoelscher
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel E Kaufmann, Bruce D Walker
Dec 1, 2010·The Journal of Experimental Medicine·Christof GeldmacherRichard A Koup
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheryl L DayWillem A Hanekom
Jan 8, 2013·The Journal of Infectious Diseases·Andreia P SoaresWillem A Hanekom

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

FlowJo
Treestar
GraphPad Prism

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