Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein

DNA Repair
Agnès TissierAgnès Cordonnier

Abstract

The progress of replicative DNA polymerases along the replication fork may be impeded by the presence of lesions in the genome. One way to circumvent such hurdles involves the recruitment of specialized DNA polymerases that perform limited incorporation of nucleotides in the vicinity of the damaged site. This process entails DNA polymerase switch between replicative and specialized DNA polymerases. Five eukaryotic proteins can carry out translesion synthesis (TLS) of damaged DNA in vitro, DNA polymerases zeta, eta, iota, and kappa, and REV1. To identify novel proteins that interact with hpol eta, we performed a yeast two-hybrid screen. In this paper, we show that hREV1 interacts with hpol eta as well as with hpol kappa and poorly with hpol iota. Furthermore, cellular localization analysis demonstrates that hREV1 is present, with hpol eta in replication factories at stalled replication forks and is tightly associated with nuclear structures. This hREV1 nuclear localization occurs independently of the presence of hpol eta. Taken together, our data suggest a central role for hREV1 as a scaffold that recruits DNA polymerases involved in TLS.

References

Jul 10, 1999·Science·R E JohnsonL Prakash
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·V L GerlachE C Friedberg
Oct 28, 1999·Nucleic Acids Research·W LinZ Wang
Nov 11, 1999·Mutation Research·A M Cordonnier, R P Fuchs
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·P E GibbsV M Maher
Nov 30, 2000·Nucleic Acids Research·Y ZhangZ Wang
Feb 13, 2001·Nucleic Acids Research·Y ZhangZ Wang
Aug 4, 2001·The Journal of Biological Chemistry·Y MurakumoM Takahashi
Aug 22, 2003·Nucleic Acids Research·Denise R ClarkW Glenn McGregor
Jan 17, 2004·Biochimie·P Kannouche, A Stary
Feb 28, 2004·The Journal of Biological Chemistry·Thomas A Kunkel

❮ Previous
Next ❯

Citations

Jul 27, 2007·Cell Biochemistry and Biophysics·Laura R BarkleyCyrus Vaziri
Nov 27, 2008·Cell Biochemistry and Biophysics·Magda Budzowska, Roland Kanaar
Jun 14, 2005·DNA Repair·Vladimir PoltoratskySamuel H Wilson
Dec 25, 2007·Cell Research·Gregory N GanRichard D Wood
Jan 17, 2009·Nature Chemical Biology·Debbie J Chang, Karlene A Cimprich
Dec 13, 2005·Nature Reviews. Molecular Cell Biology·Errol C Friedberg
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Tony T Huang, Alan D D'Andrea
Feb 24, 2012·Nature Reviews. Molecular Cell Biology·Julian E SaleRoger Woodgate
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lauren S Waters, Graham C Walker
Jul 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jürgen BachlBerit Jungnickel
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei Yang, Roger Woodgate
Oct 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Atsuko NiimiAlan R Lehmann
Jun 16, 2012·The Journal of Biological Chemistry·Jessica WojtaszekPei Zhou
Feb 16, 2006·The Journal of Experimental Medicine·Jacob G JansenNiels de Wind
May 10, 2011·Molecular Biology of the Cell·Parker L AndersenWei Xiao
Jan 18, 2005·Nucleic Acids Research·Jacob G JansenNiels de Wind
Dec 21, 2012·Nucleic Acids Research·Myoung Shin KimYuichi J Machida
Nov 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Julian E SaleDávid Szüts
Mar 6, 2012·Molecular Pharmacology·Shilpy SharmaChristine E Canman
Apr 1, 2009·Molecular and Cellular Biology·Jacob G JansenNiels de Wind
Apr 22, 2009·Molecular and Cellular Biology·Laurie ReyJean-Sébastien Hoffmann
Aug 23, 2006·Molecular and Cellular Biology·Sanjay D'Souza, Graham C Walker
Sep 20, 2006·Molecular and Cellular Biology·Caixia GuoErrol C Friedberg
Sep 19, 2007·Molecular and Cellular Biology·Narottam AcharyaLouise Prakash
Jun 22, 2011·Molecular and Cellular Biology·Franklin Mayca PozoTakayuki Yamashita
Oct 18, 2005·Molecular and Cellular Biology·Narottam AcharyaLouise Prakash
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Lauren S WatersGraham C Walker
Jun 15, 2005·Annual Review of Biochemistry·Satya PrakashLouise Prakash
Jan 30, 2009·Molecular Cancer Research : MCR·Chad A DumstorfW Glenn McGregor
Aug 31, 2007·BMC Genomics·Raj ChariStephen Lam
Feb 21, 2008·BMC Molecular Biology·Jan BrunDouglas A Gray
Nov 4, 2011·Cancer Cell International·Philip A Knobel, Thomas M Marti
Jun 23, 2006·PLoS Genetics·Roland K ChiuBradly G Wouters
Oct 29, 2010·Genetics·Mary Ellen Wiltrout, Graham C Walker
Oct 28, 2006·Radiation Research·Joann B SweasyKristin A Eckert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.