Co-localization of somatostatin mRNA and parvalbumin in the dorsal rat hippocampus after cerebral ischemia

Hippocampus
R BeringF F Johansen

Abstract

Following transient global ischemia most of the neurons containing somatostatin in the fascia dentata of the dorsal hippocampal formation die, while somatostatinergic neurons in the CA1 region survive. The neurons react to ischemia with a transiently reduced expression of somatostatin mRNA and peptide. We have tested the hypothesis that this selective vulnerability is solely related to those somatostatinergic neurons which do not express the calcium-binding protein parvalbumin. Postischemic changes were studied in rat dorsal hippocampus at 2 and 16 days after 10 min of global cerebral ischemia using a four-vessel occlusion model. We performed a double-staining visualizing the mRNA coding for somatostatin by non-radioactive in situ hybridization and parvalbumin protein by immunocytochemistry. Only 5% of the somatostatinergic cells in the fascia dentata contained parvalbumin. The number of somatostatinergic cells was permanently reduced following ischemia. Among surviving neurons we found cells with and without parvalbumin expression. Thus, expression of parvalbumin is not predictive for survival of somatostatinergic cells in the fascia dentata. In contrast, in CA1, 37% of the somatostatinergic cells contained parvalbumin. These ...Continue Reading

References

May 1, 1979·Stroke; a Journal of Cerebral Circulation·W A Pulsinelli, J B Brierley
Jul 1, 1991·Brain Research. Molecular Brain Research·A RepresaY Ben-Ari
Oct 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H Benveniste, N H Diemer
Aug 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J P PetraliL A Sternberger
Jan 1, 1980·CRC Critical Reviews in Biochemistry·R H Kretsinger
Nov 1, 1982·Acta Neurologica Scandinavica·M B Jørgensen, N H Diemer
Mar 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T MatsuyamaM Sugita
Dec 1, 1993·The Journal of Physiology·P S ChardR J Miller

❮ Previous
Next ❯

Citations

Aug 15, 2006·Experimental Brain Research·Georg Johannes MüllerFlemming Fryd Johansen
Aug 20, 2014·Experimental Neurology·Georg Johannes MüllerFlemming Fryd Johansen
May 10, 1995·Annals of the New York Academy of Sciences·B S Meldrum
Jan 9, 2007·Neurobiology of Disease·Georg Johannes MüllerFlemming Fryd Johansen
Sep 7, 2004·The Journal of Comparative Neurology·Christiane FrahmOtto W Witte
Sep 8, 2006·The Journal of Comparative Neurology·Xiangmin XuEdward M Callaway
Jul 21, 2004·Neuroscience·C FrahmO W Witte
Dec 21, 2004·Brain Pathology·Georg Johannes MüllerFlemming Fryd Johansen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.