Co-localization of the D1 dopamine receptor in a subset of DARPP-32-containing neurons in rat caudate-putamen

Neuroscience
K C LangleyC C Ouimet

Abstract

DARPP-32 (dopamine- and cyclic AMP-regulated phosphoprotein, apparent molecular weight of 32,000) is part of the D1 dopamine receptor signal transduction cascade. Both the D1 receptor and DARPP-32 are found in the caudate putamen, but it is not known if they co-localize in the medium-sized spiny neurons. In the present study, double-labelling immunocytochemistry was used to simultaneously localize the D1 receptor and DARPP-32 in the rat caudate-putamen. The neuropil was heavily and uniformly immunoreactive for both the D1 receptor and DARPP-32. All cell bodies immunopositive for the D1 receptor were immunopositive for DARPP-32. The D1 receptor was not detectable, however, in nearly half of the DARPP-32-containing cell bodies. DARPP-32 is present in striatopallidal and striatonigral projections. The D1 receptor co-localized with DARPP-32 in fibres of the entopeduncular nucleus and the pars reticulata of the substantia nigra. In the globus pallidus, however, D1 receptor immunoreactivity was barely detectable, while DARPP-32 immunolabelling of axons and axon terminals was intense. These data suggest that the striatal somata containing both the D1 receptor and DARPP-32 project to the entopeduncular nucleus and substantia nigra, whe...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J SurmeierS T Kitai
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S FerreK Fuxe
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M WeinerM R Brann
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C Le MoineB Bloch
Aug 18, 1991·The Journal of Comparative Neurology·E L GustafsonP Greengard
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F J MonsmaD R Sibley
May 1, 1983·Neuroscience·M A Ariano, S K Ufkes
Jan 1, 1994·Trends in Neurosciences·B Bloch, C Le Moine
Aug 1, 1993·Trends in Neurosciences·D J SurmeierM A Ariano
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K TsouP Greengard
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A I LeveyB J Ciliax

❮ Previous
Next ❯

Citations

Jul 4, 1998·Brain Research. Brain Research Reviews·P GreengardA Nishi
Dec 22, 1998·Brain Research. Brain Research Reviews·A ReinerC L Veenman
Sep 29, 1999·Progress in Neurobiology·P SvenningssonB B Fredholm
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·P SvenningssonG Fisone
Apr 10, 2008·PloS One·Stephen C GammieSharon A Stevenson
Sep 24, 2013·PloS One·Hans BlomHjalmar Brismar
Dec 10, 2015·Frontiers in Neuroanatomy·Ryoma Morigaki, Satoshi Goto
May 24, 2000·Brain Research·C M ArakiL R Britto
Jun 14, 2003·The Journal of Pharmacology and Experimental Therapeutics·Shao-Pii OnnAnthony A Grace
Jun 16, 2010·Pharmacology & Therapeutics·Ashiwel S Undieh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.