Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium

Biotechnology Letters
Ju-Hyun Yu, A M Klibanov

Abstract

The impact of co-lyophilizing horseradish peroxidase (HRP) with numerous amino acids and other chiral excipients on the enzyme's subsequent stereoselectivity [E(S/R)] in the sulfoxidation of thioanisole in 2-propanol was systematically investigated. While many improved the stereoselectivity of (and significantly activated) HRP, the greatest effect was observed with D-proline which enhanced the E(S/R) value by over an order of magnitude from synthetically meaningless to useful.

References

Apr 13, 1995·Nature·A M Klibanov
Feb 6, 1999·Advances in Biochemical Engineering/biotechnology·W AdamP Schreier
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·L Dai, A M Klibanov
Sep 19, 2000·Biotechnology and Bioengineering·L Dai, A M Klibanov
Feb 24, 2001·Nature·A M Klibanov
Jan 31, 2002·Journal of the American Chemical Society·Prasanta Kumar DasAlexander M Klibanov
Aug 29, 2003·Current Opinion in Biotechnology·Alexander M Klibanov
Jun 27, 2007·Biotechnology and Bioengineering·Yuchun XieAlexander M Klibanov

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Citations

Jul 31, 2014·Applied Microbiology and Biotechnology·Toru MatsuiMakoto Ueda
Jan 13, 2015·Applied Microbiology and Biotechnology·Florian W Krainer, Anton Glieder
Apr 27, 2010·Chemical Reviews·Elzbieta Wojaczyńska, Jacek Wojaczyński

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