PMID: 11316258Apr 24, 2001Paper

Co-operativity between mouse connexin30 gap junction channels

Pflügers Archiv : European journal of physiology
V Valiunas, R Weingart

Abstract

HeLa cells stably transfected with mouse cDNA coding for connexin30 (Cx30) were used to study the electrical properties of gap junction channels. The experiments involved the measurement of intercellular currents (Ij) from cell pairs using dual whole-cell recording with the patch-clamp method. The aim was to compare Ij from cell pairs whose gap junctions consisted of a single channel and cell pairs whose gap junctions consisted of many channels. We found that both the ensemble average currents gained from single-channel records and the currents obtained from multichannel records inactivated exponentially with time. However, the former inactivated significantly slower than the latter. At ajunctional voltage (Vj) of 50 mV, the time constants of inactivation (tau(i)) were 8.1 s and 1.6 s, respectively. Moreover, the ratio tau(i)(single-channel)/tau(i)(multichannel) turned out to be voltage sensitive, i.e. it decreased with increasing V(j) These observations suggest that the operation of Cx30 gap junction channels in the multichannel configuration involves co-operative interactions.

Citations

Sep 5, 2002·Journal of Cellular Biochemistry·Udo B ZacharzowskyIngo Morano
Nov 12, 2005·The Journal of Membrane Biology·S V RamananP R Brink
Nov 9, 2006·The Journal of Membrane Biology·Francisco J Martinez-WittinghanRichard T Mathias
Nov 6, 2009·American Journal of Physiology. Cell Physiology·Mauricio A RetamalLuis Reuss
Apr 26, 2006·Circulation Journal : Official Journal of the Japanese Circulation Society·Susumu TakeuchiItsuo Kodama
Aug 30, 2005·Bioelectrochemistry·Paramita Ghosh, Subhendu Ghosh

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