Co-production of vascular endothelial cadherin and inducible nitric oxide synthase by endothelial cells in periapical granuloma

International Endodontic Journal
S HamaK Ito

Abstract

To clarify the mechanisms of inflammatory cell migration in human periapical granulomas by examining vascular endothelial (VE) cadherin and inducible nitric oxide synthase (iNOS)-producing cells. Periapical tissues were obtained from patients during endodontic surgery and were divided into two portions. After fixing the tissues with acetone or 4% paraformaldehyde in phosphate-buffered saline, 5-microm-thick paraffin or cryostat sections were prepared, respectively. The paraffin sections of the inflamed tissues were evaluated histologically with haematoxylin-eosin stains. Cryostat sections of the tissue, diagnosed as periapical granulomas, were then examined by either immunohistochemistry using anti-human VE-cadherin or iNOS antibodies (Abs) for the characterization of infiltrating cells. In addition, co-localization of VE-cadherin and iNOS production was also analysed by two-colour immunofluorescence image analysis. Endothelial cells were strongly stained with iNOS Abs. Macrophages, lymphocytes, polymorphonuclear leucocytes and fibroblasts also exhibited iNOS production. These iNOS-positive cells accumulated around the blood vessels. On the other hand, VE-cadherin production was exhibited in only endothelial cells. Two-colour i...Continue Reading

References

Feb 1, 1989·Endodontics & Dental Traumatology·M Haapasalo
Nov 1, 1995·Journal of Endodontics·K YanagiguchiH Okabe
May 13, 1999·International Endodontic Journal·P N Nair
Jul 17, 2001·Immunology·R J van't Hof, S H Ralston
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jaap D van BuulPeter L Hordijk
Apr 23, 2003·Biochemical and Biophysical Research Communications·D GonzálezA Rojas
Mar 27, 2004·Journal of Dental Research·F Di Nardo Di MaioM Felaco
Sep 18, 2004·European Journal of Immunology·Britta Engelhardt, Hartwig Wolburg
Oct 27, 2004·Investigative Ophthalmology & Visual Science·Pervinder SagooAndrew J T George

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Citations

May 5, 2009·Journal of Endodontics·Malte SchulzDieter Bosshardt
Aug 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Raquel Assed Bezerra da SilvaAlexandra Ivo de Medeiros
Oct 15, 2009·International Endodontic Journal·R A S S RochaP Nelson-Filho

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