Abstract
Birc5 (previously known as survivin) is a cancer-specific protein. Due to the upregulation of its expression in various human malignancies and its key role in apoptosis, proliferation and angiogenesis, Birc5 has attracted attention as a target for anticancer therapies. In this study, when Birc5 was silenced in HepG2 cells, 29.7±3.3% cells underwent apoptosis as expected. It was found that the expression levels of glucose-regulated protein 78 (Hspa5, previously known as Grp78) was increased by almost 3-fold in Birc5-silenced HepG2 cells. Hspa5, a master regulator of the anti-apoptotic unfolded protein response signalling network, can also promote tumor proliferation, survival and metastasis. Hence, we hypothesized that the co-silencing of Birc5 and Hspa5 may exert a stronger apoptosis-inducing effect than single gene interference. To verify this, the expression levels of Birc5 and Hspa5 in human hepatocellular carcinoma tissues were determined. Immunohistochemical staining showed that the expression of Birc5 and Hspa5 was elevated in 28 out of 31 samples. Additionally, plasmid-based siRNA against Birc5 and/or Hspa5 were constructed and transfected into the human hepatocellular liver carcinoma cell line, HepG2. Compared with the ...Continue Reading
References
Jan 2, 2003·Nature Reviews. Cancer·Dario C Altieri
Apr 1, 2003·The Journal of Biological Chemistry·Ramachandra K ReddyAmy S Lee
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paola FortugnoDario C Altieri
Nov 25, 2003·Oncogene·Dario C Altieri
Jan 16, 2004·Oncogene·Marzia PennatiNadia Zaffaroni
Jan 24, 2004·Cancer Gene Therapy·Matthias KapplerHelge Taubert
May 1, 2004·Cell Death and Differentiation·M Chawla-SarkarE C Borden
Dec 4, 2004·International Journal of Cancer. Journal International Du Cancer·Fengzhi LiDongfeng Tan
Nov 22, 2005·Cancer Detection and Prevention·Qi WangYuhua Chen
Feb 14, 2006·Current Molecular Medicine·Jianze Li, Amy S Lee
Apr 19, 2006·Cancer Research·Elena TirròPaolo Vigneri
Aug 3, 2006·Journal of the National Cancer Institute·Boglarka GyurkoczaDario C Altieri
Feb 8, 2007·British Journal of Cancer·A R HinnisR A Walker
Mar 8, 2007·Carcinogenesis·Marzia PennatiNadia Zaffaroni
Apr 12, 2007·Cancer Biology & Therapy·Hongyun LiuPeixuan Guo
Aug 11, 2007·The International Journal of Biochemistry & Cell Biology·M WangG X Shen
Sep 7, 2007·Cancer Research·Takahito NakaharaMasao Sasamata
Jul 4, 2008·Cell Stress & Chaperones·Min WangGuan-Xin Shen
Oct 11, 2008·International Journal of Cancer. Journal International Du Cancer·Wen-Jie HuangUNKNOWN De-AnTian
May 28, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julian A SchardtThomas Pabst
Aug 29, 2009·Blood·Johann KernMichael Steurer
Sep 1, 2009·PloS One·Min NiAmy S Lee
Oct 14, 2009·Molecular BioSystems·Li LiGuanxin Shen
Dec 18, 2009·Proteomics·Serge ArnaudeauMarie Cohen
Mar 10, 2010·Current Drug Targets·Eishi AshiharaTaira Maekawa
Apr 15, 2010·Nucleosides, Nucleotides & Nucleic Acids·Puja SapraIvan D Horak
Apr 20, 2010·Molecular Cancer·Chun Hei Antonio CheungJang-Yang Chang
Sep 18, 2010·Neoplasma·Y F WuH Y Shen
Jan 6, 2011·Cancer Science·Takahito NakaharaMasao Sasamata
Jan 11, 2011·Molecular Cancer Therapeutics·Rosa A CarrascoBharvin K R Patel
Feb 19, 2011·Methods in Enzymology·Sakae Saito, Akihiro Tomida
Jul 27, 2011·Neuro-oncology·Sandy HendruschkAchim Temme
Citations
Sep 6, 2014·International Journal of Molecular Sciences·Angelica Judith Granados López, Jesús Adrián López
May 29, 2016·Chemico-biological Interactions·Xiaoxue ZhangGuanxin Shen
Jun 1, 2016·PloS One·Ashok PalaniappanSatish Ramalingam
Jun 12, 2013·International Journal of Molecular Sciences·Doreen KunzeSusanne Fuessel
Oct 19, 2017·Molecular Medicine Reports·Mingjing XuGuozhi Huang
Oct 24, 2014·Molecular Medicine Reports·Tong LuZhangqun Ye
Dec 14, 2018·Molecular and Clinical Oncology·Qiandong ZhuHaixin Qian
Jul 10, 2018·Oncotarget·Qing JiangGuanxin Shen
Nov 3, 2020·Frontiers in Immunology·Lu ChenGuanxin Shen
May 3, 2021·European Journal of Medicinal Chemistry·Meizhu WangWen Zhou
Jun 21, 2013·Journal of Proteome Research·Hiroshi IchikawaTadashi Kondo