Co-suppression of AtMIPS demonstrates cooperation of MIPS1, MIPS2 and MIPS3 in maintaining myo-inositol synthesis

Plant Molecular Biology
Christine M FleetGlenda E Gillaspy

Abstract

Co-suppressed MIPS2 transgenic lines allow bypass of the embryo lethal phenotype of the previously published triple knock-out and demonstrate the effects of MIPS on later stages of development. Regulation of inositol production is of interest broadly for its effects on plant growth and development. The enzyme L-myo-inositol 1-phosphate synthase (MIPS, also known as IPS) isomerizes D-glucose-6-P to D-inositol 3-P, and this is the rate-limiting step in inositol production. In Arabidopsis thaliana, the MIPS enzyme is encoded by three different genes, (AtMIPS1, AtMIPS2 and AtMIPS3), each of which has been shown to produce proteins with biochemically similar properties but differential expression patterns. Here, we report phenotypic and biochemical effects of MIPS co-suppression. We show that some plants engineered to overexpress MIPS2 in fact show reduced expression of AtMIPS1, AtMIPS2 and AtMIPS3, and show altered vegetative phenotype, reduced size and root length, and delayed flowering. Additionally, these plants show reduced inositol, increased glucose levels, and alteration of other metabolites. Our results suggest that the three AtMIPS genes work together to impact the overall synthesis of myo-inositol and overall inositol hom...Continue Reading

References

Nov 25, 2003·Bioinformatics·Anthony L DuranLloyd W Sumner
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Alex M MurphyDavid E Hanke
Apr 3, 2009·Plant Physiology·Javad TorabinejadGlenda E Gillaspy
Jun 3, 2010·The Journal of Biological Chemistry·Hao Chen, Liming Xiong
Apr 27, 2012·The Biochemical Journal·Peter Hedden, Stephen G Thomas
Jan 24, 2013·Nucleic Acids Research·David LatrasseMoussa Benhamed
Jun 7, 2015·The Plant Cell·Quentin BruggemanMarianne Delarue

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