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Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami

bioRxiv

Dec 5, 2019

Emanuela TorelliNatalio Krasnogor

Abstract

The scaffolded origami technique has provided an attractive tool for engineering nucleic acid nanostructures. This paper demonstrates scaffolded RNA origami folding in vitro in which all components are transcribed simultaneously in a single-pot reaction. Double-stranded DNA sequences ar...read more

Mentioned in this Paper

Fluorescent Dyes
In Vivo
Transcription Cofactor Activity
Transcription, Genetic
Dyes
Microscopy, Atomic Force
Green Fluorescent Proteins
Analysis
Nucleic Acids
Nucleic Acid Strand
8
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Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami

bioRxiv

Dec 5, 2019

Emanuela TorelliNatalio Krasnogor

DOI: 10.1101/864678

Abstract

The scaffolded origami technique has provided an attractive tool for engineering nucleic acid nanostructures. This paper demonstrates scaffolded RNA origami folding in vitro in which all components are transcribed simultaneously in a single-pot reaction. Double-stranded DNA sequences ar...read more

Mentioned in this Paper

Fluorescent Dyes
In Vivo
Transcription Cofactor Activity
Transcription, Genetic
Dyes
Microscopy, Atomic Force
Green Fluorescent Proteins
Analysis
Nucleic Acids
Nucleic Acid Strand
8

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BioRxiv Preprints

BioRxiv is the preprint server for biology, operated by Cold Spring Harbor Laboratory. Here are the latest biology preprint articles from bioRxiv.

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Isothermal folding of a light-up bio-orthogonal RNA origami nanoribbon

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