Co-vaccination with adeno-associated virus vectors encoding human papillomavirus 16 L1 proteins and adenovirus encoding murine GM-CSF can elicit strong and prolonged neutralizing antibody

International Journal of Cancer. Journal International Du Cancer
Dai-Wei LiuShow-Li Chen

Abstract

Non-infectious human papillomavirus-like particles (VLPs), encoded by the major capsid gene L1, have been shown to be effective as vaccines to prevent cervical cancer. We have developed the genetic immunization of the L1 gene to induce a neutralizing antibody. We constructed and generated a recombinant adeno-associated virus encoding human papillomavirus (HPV) 16 L1 protein that could form virus-like particles in transduced cells. Previous reports have demonstrated that the formation of VLP is necessary to induce high titers of neutralizing antibodies to protect an animal from viral challenge. Therefore, we carried out a single intramuscular (i.m.) injection with recombinant adeno-associated virus encoding HPV-16 L1 protein (rAAV-16L1) in BALB/c mice, which ultimately produced stronger and more prolonged neutralizing L1 antibodies, when compared to the DNA vaccine. Immunohistochemistry showed that the accumulation of antigen presenting cells, such as macrophages and dendritic cells, in rAAV-16L1 and L1 DNA-injected muscle fibers may be due to the L1 protein expression, but not to AAV infection. When compared to the L1 VLP vaccine, however, the titers of neutralizing L1 antibodies induced by VLP were higher than those induced by...Continue Reading

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Aug 12, 2005·Current Opinion in Oncology·Anna S Kadish, Mark H Einstein
Feb 2, 2012·Antiviral Therapy·Elizabeth D GerschRobert L Garcea
Oct 1, 2008·The Journal of Adolescent Health : Official Publication of the Society for Adolescent Medicine·Anna-Barbara Moscicki
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Sep 27, 2018·Frontiers in Immunology·Cleo Goyvaerts, Karine Breckpot

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