CO2 to Terpenes: Autotrophic and Electroautotrophic α-Humulene Production with Cupriavidus necator

Angewandte Chemie
Thomas KriegDirk Holtmann

Abstract

We show that CO2 can be converted by an engineered "Knallgas" bacterium (Cupriavidus necator) into the terpene α-humulene. Heterologous expression of the mevalonate pathway and α-humulene synthase resulted in the production of approximately 10 mg α-humulene per gram cell dry mass (CDW) under heterotrophic conditions. This first example of chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen, and oxygen is a promising starting point for the production of different high-value terpene compounds from abundant and simple raw materials. Furthermore, the production system was used to produce 17 mg α-humulene per gram CDW from CO2 and electrical energy in microbial electrosynthesis (MES) mode. Given that the system can convert CO2 by using electrical energy from solar energy, it opens a new route to artificial photosynthetic systems.

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Citations

May 1, 2018·Applied Microbiology and Biotechnology·Jayashree Chakravarty, Christopher J Brigham
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Feb 23, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Wissam FarhatPer-Olof Syrén
Mar 24, 2020·Angewandte Chemie·Ammar Al-ShameriLars Lauterbach
Jul 13, 2019·Biotechnology for Biofuels·Carina Windhorst, Johannes Gescher
Oct 1, 2020·ChemSusChem·Anthony J Abel, Douglas S Clark
Apr 25, 2020·Angewandte Chemie·Frank HollmannCaroline E Paul
Feb 2, 2021·Trends in Biotechnology·Justin PanichSteven W Singer
Nov 16, 2019·New Biotechnology·Lucile GarriguesStéphane E Guillouet
Apr 28, 2021·Microbial Cell Factories·Sofia Milker, Dirk Holtmann
Apr 13, 2021·Biotechnology and Bioengineering·Sofia MilkerDirk Holtmann
Jun 27, 2021·Nature Reviews. Microbiology·Jay KeaslingEric Sundstrom
Jul 6, 2021·Metabolic Engineering·Joshua Luke GascoyneNaglis Malys
Aug 18, 2020·Metabolic Engineering·Nico J ClaassensArren Bar-Even
Nov 10, 2021·Journal of Agricultural and Food Chemistry·Qi GuoHe Huang

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