Coadministration of the cyanobacterial lipopolysaccharide antagonist CyP with antibiotic inhibits cytokine production by an in vitro meningitis model infected with Neisseria meningitidis

The Journal of Antimicrobial Chemotherapy
Rebecca OliverM Christodoulides

Abstract

In this study, the objective was to determine the anti-inflammatory properties of CyP, a cyanobacterial lipopolysaccharide (LPS) antagonist, used in combination with antibiotic chemotherapy during infection of an in vitro meningitis model infected with Neisseria meningitidis (meningococcus). Monocultures of human meningioma cells and meningioma-primary human macrophage co-cultures were infected with meningococci (10(2)-10(8) cfu/monolayer) or treated with isolated outer membranes or purified LPS (0.1-100 ng/monolayer) from N. meningitidis. CyP (1-20 μg/monolayer) was added at intervals from t = 0 to 4 h, with and without benzylpenicillin (1-20 μg/monolayer). The antagonistic effect of CyP and its adjunctive properties to benzylpenicillin administration was determined by measuring cytokine levels in culture supernatants after 24 h. CyP significantly inhibited (P < 0.05) the secretion of interleukin (IL)-6, IL-8, monocyte chemoattractant protein (MCP)-1 and RANTES ('regulated upon activation, normal T cell expressed and secreted') (overall reduction levels from 50% to >95%) by meningioma cell lines and meningioma-macrophage co-cultures challenged with either live meningococci or bacterial components. Inhibition was effective when...Continue Reading

References

Aug 25, 1977·The New England Journal of Medicine·J P Hieber, J D Nelson
Feb 1, 1989·The Journal of Infectious Diseases·P BrandtzaegE Sørensen
Oct 1, 1982·The Journal of Infectious Diseases·D S StephensZ A McGee
Jan 1, 1982·Applied and Environmental Microbiology·G Keleti, J L Sykora
Apr 7, 1995·Science·W J ChristM A Mullarkey
Feb 1, 1993·The Journal of Infectious Diseases·A HalstensenA Waage
Apr 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L F López-CortésE Jiménez
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Jan 11, 2000·Clinical Microbiology Reviews·M van DeurenJ W van der Meer
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PoltorakB Beutler
Jun 9, 2000·Molecular Microbiology·S J HardyJ E Heckels
Dec 26, 2001·Journal of Endotoxin Research·P BrandtzaegP Kierulf
May 28, 2004·Cell·Patrick J Lupardus, Karlene A Cimprich
May 24, 2006·The Journal of Experimental Medicine·Annalisa MacagnoFederica Sallusto
Jan 1, 1993·Mediators of Inflammation·A WaageP Brandtzæg
Aug 9, 2008·International Journal of Cancer. Journal International Du Cancer·Maria A TichomirowaUlrich Renner
Aug 8, 2009·Critical Care Medicine·Mark TidswellUNKNOWN Eritoran Sepsis Study Group
Jul 12, 2011·Current Biology : CB·Eva Marie Y MorescoBruce Beutler

❮ Previous
Next ❯

Citations

Mar 19, 2014·Carbohydrate Research·Sara CarilloMaria Michela Corsaro
Jul 23, 2015·Marine Drugs·Prasannavenkatesh DuraiSangdun Choi
Sep 19, 2012·Expert Opinion on Pharmacotherapy·Seon Hee Shin, Kwang Sik Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Meningitis

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.

Bacterial Meningitis (ASM)

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.