Coagulansin-A has beneficial effects on the development of bovine embryos in vitro via HSP70 induction

Bioscience Reports
Imran KhanIl-Keun Kong

Abstract

Coagulansin-A (withanolide) is the steroidal lactone obtained from Withania coagulans which belong to Solanaceae family. The present study investigated the effects of coagulansin-A on bovine oocyte maturation and embryo development in vitro. All these oocytes were aspirated from the ovaries obtained from Korean Hanwoo cows at a local abattoir. To determine whether coagulansin-A has beneficial effects on bovine oocyte maturation in vitro, 355 oocytes per group (control and treated) in seven replicates were subjected with different concentrations (1, 2.5, 5, 7.5 and 10 μM) of coagulansin-A. The coagulansin-A was added in the in vitro maturation (IVM) media followed by in vitro fertilization (IVF) and then in vitro culture (IVC). Only treatment with 5 μM coagulansin-A remarkably (P<0.05) improved embryos development (Day 8 blastocyst) having 27.30 and 40.01% for control and coagulansin-A treated groups respectively. Treatment with 5 μM coagulansin-A significantly induced activation of heat shock protein 70 (HSP70) (P<0.05). Immunofluorescence analysis revealed that 5 μM coagulansin-A treatment also significantly inhibited oxidative stress and inflammation during bovine embryo development in vitro by decreasing 8-oxoguanosine (8-Ox...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·R J Ellis, S M van der Vies
Jan 1, 1991·Molecular Reproduction and Development·J Hendrey, I Kola
Jan 1, 1989·Chemico-biological Interactions·M Comporti
Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Nov 30, 1994·Experientia·M R Jacquier-SarlinB S Polla
Nov 1, 1993·Journal of Reproduction and Fertility·B Fischer, B D Bavister
Sep 1, 1993·Biology of Reproduction·C F RosenkransN L First
Jan 1, 1997·Reviews of Reproduction·E de LamirandeC Gagnon
May 18, 1999·Biology of Reproduction·A NishikimiM Yamada
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Jul 31, 2003·Reproduction in Domestic Animals = Zuchthygiene·P LonerganM P Boland
Aug 20, 2004·Human Molecular Genetics·Toshio HamataniMinoru S H Ko
May 26, 2006·Biochemical Pharmacology·Geoffrey GloireJacques Piette
Jun 30, 2006·Nucleic Acids Research·Alison ZimonAnny Usheva
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Osman V PatelGeorge W Smith
May 1, 2007·Theriogenology·A Van SoomL Peelman
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Yang YuWeizhi Ji
Feb 2, 2008·Human Reproduction Update·Carla TatoneRiccardo Focarelli
Feb 13, 2008·Cell·Matthew S Hayden, Sankar Ghosh
Feb 10, 2009·Clinical Science·Ee Tsin Wong, Vinay Tergaonkar
May 22, 2010·The Journal of Pharmacy and Pharmacology·Rakesh Maurya
Jan 16, 2013·Journal of Natural Products· Ihsan-ul-HaqLeng Chee Chang
Aug 28, 2014·Biochemical Pharmacology·Karen HeyninckWim Vanden Berghe

❮ Previous
Next ❯

Citations

Dec 28, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Yunwei PangHuabin Zhu
Feb 15, 2017·Reproduction, Fertility, and Development·Imran KhanIl-Keun Kong
Dec 9, 2017·Molecular Reproduction and Development·M M R ChowdhuryIl-Keun Kong
Jan 11, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Imran KhanIl-Keun Kong
Jun 2, 2017·Animal Genetics·M F Suqueli GarcíaP M Corva

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
PCR

Software Mentioned

Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.