PMID: 6404055Mar 1, 1983Paper

Coat protein binding sites on RNA 1 of alfalfa mosaic virus

Virology
D ZuidemaE M Jaspars

Abstract

The largest genome segment, RNA 1, of alfalfa mosaic virus forms complexes with viral coat protein. These complexes were subjected to digestion with ribonucleases T1 or A and filtered onto Millipore filters. Specific fragments were collected from the filters by phenol extraction. After electrophoretic separation in denaturing polyacrylamide gels, these fragments were sequenced. Besides extracistronic fragments originating from the 3'-terminal region of RNA 1, fragments were found originating from an intracistronic region of the RNA. A striking phenomenon is that the intracistronic fragments were not found when ribonuclease A was used to degrade RNA/protein complexes. The findings are in agreement with the postulation of Houwing and Jaspars (1978), that a conformational change at the 3' ends of the genome RNAs induced by the coat protein is a prerequisite to start an infection cycle.

References

Jul 11, 1978·Biochemistry·C J Houwing, E M Jaspars
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E C Koper-Zwarthoff, J F Bol
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E C Koper-ZwarthoffJ F Bol
Aug 1, 1977·Nucleic Acids Research·H Donis-KellerW Gilbert
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Jul 6, 1982·Biochemistry·C J Houwing, E M Jaspars
May 24, 1980·Nucleic Acids Research·F T BrederodeJ F Bol
Nov 11, 1980·Biochemistry·C J Houwing, E M Jaspars

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Citations

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·W ZhongR Rueckert
Mar 12, 1984·Nucleic Acids Research·B J CornelissenJ F Bol
Feb 28, 2001·Journal of Personality and Social Psychology·R M Sutton, J McClure
Dec 1, 1989·Journal of Virology·B WeissS Schlesinger

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